Vitamin Mixture Solution

Vitamin mixtures are used regularly in promoting growth of bacteria and fungi from different types of microbiomes, such as gut, rumen, soil, water, etc[1],[2]–[3]. The use of vitamin mixtures helps scientists reduce time spent on creating the optimal conditions for microbial growth of a specific bacteria as well as diversified culturing of microbial samples. Different types of bacteria (aerobic, anaerobic, extremophiles, etc.) require different combinations of a variety of vitamins to improve their culturability. These include water-soluble vitamins, such as the B vitamins which are a broad category of small molecules that are important for cell metabolism but otherwise do not necessarily share structural or functional characteristics[4]. In microbial studies the use of sequencing techniques is very common for identification of microorganisms in a certain habitat or niche. However, there is a discrepancy between the diversity that one can identify by culture techniques and by sequencing techniques. While the diversity of bacterial species identified by NGS techniques is very high, the number of culturable bacteria and fungi is much lower. With the growth of microbial research, new efforts to optimize culture-based techniques now allow the cultivation of previously uncultivated microbiome bacteria[1],[5].

Vitamins, additives, and mineral supplements are used in special culturomics growth media that mimic the natural environment of bacteria and fungi for high throughput culturing. Vitamin mixture solutions contain nutrients and cofactors necessary to support growth from different types of microbial environments like human/animal microbiome, environmental microbiome, as well as other industries such as health and nutrition that rely on microbial research and diagnostics.