用途
sufficient for 100 colorimetric tests
质量水平
检测方法
colorimetric
储存温度
−20°C
基因信息
human ... PDHA1(5160) , PDHA2(5161 ) , PDHB(5162) , PDHX(8050)
mouse ... PDHA1(18597) , PDHA2(18598) , PDHB(68263) , PDHX(27402)
rat ... PDHA1(29554) , PDHA2(117098) , PDHB(289950) , PDHX(311254)
一般描述
Pyruvate Dehydrogenase (PDH) is a mitochondrial enzyme which has an important role in carbohydrate metabolism. Combining with dihydrolipoyl transacetylase (E2) and dihydrolipoyl dehydrogenase (E3), PDH (E1) forms a well-characterized enzyme complex (PDC). In the presence of NAD+ and CoA, PDH catalyzes the conversion of pyruvate into Acetyl-CoA and CO2. Thus, PDH links the glycolysis metabolic pathway (cytoplasm) to the citric acid cycle (mitochondria). The Pyruvate Dehydrogenase Activity Assay Kit provides a sensitive, simple, fast, direct and convenient way for the detection of PDH activity in various samples, based on a colorimetric method. In the assay, PDH activity changes a colorless developer to a colored product. The amount of the colorimetric (450 nm) product is proportional to the enzymatic activity present in the sample. The kit can detect PDH activity that is lower than 0.1 mU, in a variety of samples.
应用
Pyruvate Dehydrogenase (PDH) Activity Assay Kit has been used in the colorimetric measurement of PDH Activity in biological samples.
特点和优势
Sensitive, simple, fast, direct and convenient way for the detection of PDH activity in various biological samples.
Supportive calculator will calculate the reagents needed according to your experimental needs, and analyze your results based on your experimental data.
Click here to download a calculator excel file
Supportive calculator will calculate the reagents needed according to your experimental needs, and analyze your results based on your experimental data.
Click here to download a calculator excel file
适用性
Suitable for measuring PDH Activity in adherent or suspension cells, from an animal or plant source.
单位定义
One unit of pyruvate dehydrogenase is the amount of enzyme that will generate 1.0 µmole of NADH per minute at 37 °C at pH=7.5.
警示用语：
Danger
危险分类
Carc. 2 - Eye Irrit. 2 - Muta. 2 - Resp. Sens. 1 - Skin Irrit. 2
WGK
WGK 3
法规信息
新产品
Structural basis for inactivation of the human pyruvate dehydrogenase complex by phosphorylation: role of disordered phosphorylation loops
Kato M, et al.
Structure (Oxford, United Kingdom), 16(12), 1849-1859 (2008)
An improved spectrophotometric assay of pyruvate dehydrogenase in lactate dehydrogenase contaminated mitochondrial preparations from human skeletal muscle
Chretien D, et al.
Clinica Chimica Acta; International Journal of Clinical Chemistry, 240(2), 129-136 (1995)
A key role for mitochondrial gatekeeper pyruvate dehydrogenase in oncogene-induced senescence
Kaplon J, et al.
Nature, 498(7452), 109-112 (2013)
Rapid intramolecular coupling of active sites in the pyruvate dehydrogenase complex of Escherichia coli: mechanism for rate enhancement in a multimeric structure
Danson M J, et al.
Proceedings of the National Academy of Sciences of the USA, 75(11), 5386-5390 (1978)
Self-assembly and catalytic activity of the pyruvate dehydrogenase multienzyme complex from Bacillus stearothermophilus
Domingo G J, et al.
European Journal of Biochemistry, 266(3), 1136-1146 (1999)
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