Succinate Dehydrogenase Assay Kit

Succinate Dehydrogenase (SDH) is part of complex II found in the inner part of the mitochondrial membrane. SDH is part of the citric acid cycle (TCA) that catalyze the oxidation of succinate to fumarate. Electrons from succinate are transferred to FAD forming FADH₂ which transfer electrons to CoQ. Mutations in SDH cause hereditary paraganglioma/phaeochromocytoma syndrome and a neurodegenerative disorder known as Leigh syndrome. The Succinate Dehydrogenase Assay kit provides a simple and sensitive procedure for measuring SDH activity in a variety of tissues, cells, and isolated mitochondria. SDH activity is determined by generating a product with absorbance at 600 nm proportional to the enzymatic activity present. One unit of SDH is the amount of enzyme that generates 1.0 μmole of DCIP per minute at pH 7.2 at 25 °C.

Succinate Dehydrogenase Assay Kit has been used to measure the SDH enzyme activity in cell and tissue culture supernatants and in purified mitchondria.

• Highly sensitive, simple and high-throughput assay for measuring Succinate Dehydrogenase (SDH) concentration.
• Supportive calculator will calculate the reagents needed according to your experimental needs, and analyze your results based on your experimental data.

Click here to download a calculator excel file

Suitable for the detection of Succinate Dehydrogenase activity in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.