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检测方法
colorimetric
相关疾病
gastrointestinal diseases
储存温度
−20°C
一般描述
胰蛋白酶（EC 3.4.21.4）是一种胰腺丝氨酸蛋白酶，能够特异性剪切精氨酸和赖氨酸残基羧基侧的肽键。如果剪切位点的任意一侧存在酸性残基，则水解速率降低；如果羧基侧存在脯氨酸残基，则可能不会发生剪切。对目标蛋白质进行胰蛋白酶消化，可产生高度特异性的剪切以及数量有限的肽段。
适用性
适用于检测各种样品中的胰蛋白酶活性。
原理
在该检测中，胰蛋白酶可切割底物以产生 p-硝基苯胺（p-NA），其可在λ= 405nm处进行检测。由于颜色强度与p-NA含量成正比，因此可以对胰蛋白酶活性进行准确测定。该试剂盒可在各种样品中检测10-100mU（p-NA单位）胰蛋白酶。
警示用语：
Danger
危险声明
危险分类
Eye Dam. 1 - Resp. Sens. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
监管及禁止进口产品
A Aderibigbe et al.
Poultry science, 99(10), 5007-5017 (2020-09-30)
Trypsin inhibitors (TI) resident in soybeans affects protein utilization. While heat treatment influences residual TI, it simultaneously affects the structure and solubility of the soybean proteins and confounds any response to exogenous proteases. Using purified TI, the effect of exogenous
Montserrat Alcázar-Valle et al.
Molecules (Basel, Switzerland), 25(15) (2020-08-06)
Beans (Phaseolus spp.) are one of the most important legumes for their nutritional value and health benefits in many world regions. In addition to Phaseolus vulgaris, there are four additional species that are cultivated in many regions of the world
Sergio A Urzúa et al.
The journal of physical chemistry. B, 126(28), 5231-5240 (2022-07-13)
Under the most common experimental conditions, the adsorption of proteins to solid surfaces is a spontaneous process that leads to a rather compact layer of randomly oriented molecules. However, controlling such orientation is critically important for the development of catalytic
Ahmad Farooq et al.
Gastroenterology, 161(3), 982-995 (2021-05-30)
Heavy alcohol consumption is a common cause of acute pancreatitis; however, alcohol abuse does not always result in clinical pancreatitis. As a consequence, the factors responsible for alcohol-induced pancreatitis are not well understood. In experimental animals, it has been difficult
Ali Hanafiah Hakim et al.
Animals : an open access journal from MDPI, 12(7) (2022-04-13)
The study aimed at determining the ileal nutrient digestibility, digestive enzyme activity, intestinal morphology, and nutrient transporters mRNA expressions in broiler chickens fed with fermented PKC (LPKC) based diets with different levels of fat supplementation under hot and humid conditions.
实验方案
本方案适用于使用Nα-苯甲酰基-L-精氨酸乙酯（BAEE）作为底物测定胰蛋白酶活性。本方案是基于连续分光光度速率测定（A253，光程= 1cm）方案：
Continuous spectrophotometric rate determination method using BAEE substrate measures trypsin activity, essential for enzyme characterization.
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