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用途
sufficient for 100 colorimetric tests
检测方法
colorimetric
相关疾病
neurological disorders; cancer
储存温度
−20°C
基因信息
human ... PDHA1(5160) , PDHA2(5161) , PDHB(5162) , PDHX(8050)
mouse ... PDHA1(18597) , PDHA2(18598) , PDHB(68263) , PDHX(27402)
rat ... PDHA1(29554) , PDHA2(117098) , PDHB(289950) , PDHX(311254)
一般描述
丙酮酸脱氢酶（PDH）是一种线粒体酶，它可以催化丙酮酸转化为乙酰辅酶a和一氧化碳2，也可以连接三羧酸（TCA）和糖酵解路径。该酶被磷酸化抑制，被去磷酸化激活。PDH突变与丙酮酸脱氢酶缺乏（导致乳酸酸中毒和神经功能障碍）和莱氏症候群有关。 PDH也与癌基因诱导的衰老有关。 PDH测量可以为代谢功能和肿瘤发生提供参考。
应用
PDH活性测定试剂盒已用于测定丙酮酸脱氢酶的活性。
适用性
适于测定不同生物样品内的PDH活性，如细胞和组织培养上清液以及纯化所得的线粒体。
原理
丙酮酸脱氢酶活性可以通过偶联酶反应进行测定，生成的比色产物(450 nm)和酶促活性成正比。一个单位的丙酮酸脱氢酶是在37°C下，每分钟产生 1.0毫克分子量NADH的酶的量。
仅试剂盒组分
产品编号
说明
- PDH Assay Buffer
- PDH Substrate
- PDH Developer
- NADH Standard
- PDH Positive Control
警示用语：
Danger
危险声明
危险分类
Eye Dam. 1 - Skin Corr. 1B - Skin Sens. 1
储存分类代码
8A - Combustible corrosive hazardous materials
法规信息
动植物源性产品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
The Citric Acid Cycle Oxidizes Two-Carbon Units
Berg J M, et al.
Biochemistry (2002)
Minfeng Ying et al.
The Journal of biological chemistry, 294(24), 9615-9630 (2019-05-02)
Whether growing cancer cells prefer lactate as a fuel over glucose or vice versa is an important but controversial issue. Labeling of tricarboxylic acid (TCA) cycle intermediates with glucose or lactate isotope tracers is often used to report the relative
Ryan M Bradley et al.
Biochimica et biophysica acta. Molecular and cell biology of lipids, 1863(7), 700-711 (2018-04-09)
Lysophosphatidic acid acyltransferase (LPAAT) δ/acylglycerophosphate acyltransferase 4 is a mitochondrial enzyme and one of five homologues that catalyze the acyl-CoA-dependent synthesis of phosphatidic acid (PA) from lysophosphatidic acid. We studied skeletal muscle LPAATδ and found highest levels in soleus, a
Carine Lindquist et al.
PloS one, 14(9), e0222558-e0222558 (2019-09-25)
A fatty acid analogue, 2-(tridec-12-yn-1-ylthio)acetic acid (1-triple TTA), was previously shown to have hypolipidemic effects in rats by targeting mitochondrial activity predominantly in liver. This study aimed to determine if 1-triple TTA could influence carbohydrate metabolism. Male Wistar rats were
Pharmacological Blockade of Cannabinoid CB1 Receptors in Diet-Induced Obesity Regulates Mitochondrial Dihydrolipoamide Dehydrogenase in Muscle.
Arrabal S, et al
PLoS ONE, 10(12), e0145244-e0145244 (2015)
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