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用途
sufficient for 100 colorimetric tests
检测方法
colorimetric
相关疾病
neurological disorders; cardiovascular diseases; cancer
储存温度
2-8°C
基因信息
human ... ACO1(48) , ACO2(50)
mouse ... ACO1(11428) , ACO2(11429)
rat ... ACO1(50655) , ACO2(79250)
一般描述
该乌头酸酶是一个含铁-硫的酶家族，其催化柠檬酸异构化为异柠檬酸。人们已经在真核生物中鉴定出了由不同基因编码的两种乌头酸酶。乌头酸酶 1（ACO1，c-乌头酸酶）是一种胞质酶，而乌头酸酶 2（ACO2，m-乌头酸酶）是一种在三羧酸循环中起作用的线粒体酶。乌头碱可被氧化应激可逆地失活，并且细胞和组织中的乌头酸酶活性已被用作一种氧化损伤的生物标志物。
应用
乌头酸酶活性检测试剂盒已被用于测量细胞溶质和线粒体顺乌头酸酶的活性。
特点和优势
兼容高通量处理系统。
适用性
适用于测量组织和细胞等生物样品中的乌头酸酶活性
原理
乌头酸酶活性检测试剂盒提供了一种测量各种样品中的乌头酸酶活性的简单、直接的方法。在偶联酶反应中测定乌头酸酶活性，在该反应中柠檬酸盐被乌头酸酶转化为了异柠檬酸。因此，比色（450nm）产物与酶活性的量成正比。一个单位的乌头酸酶是指在 25℃，pH 7.4条件下每分钟将异柠檬酸异构化产生 1.0 μmole 柠檬酸盐的酶量。
警示用语：
Danger
危险声明
危险分类
Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B
储存分类代码
8A - Combustible corrosive hazardous materials
法规信息
动植物源性产品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
François Beaufay et al.
mBio, 11(4) (2020-07-30)
Maintaining cellular iron homeostasis is critical for organismal survival. Whereas iron depletion negatively affects the many metabolic pathways that depend on the activity of iron-containing enzymes, any excess of iron can cause the rapid formation of highly toxic reactive oxygen
NFS1 undergoes positive selection in lung tumours and protects cells from ferroptosis.
Alvarez S W, et al.
Nature, 551(7682), 639-639 (2017)
Jayasimman Rajendran et al.
EMBO molecular medicine, 11(1) (2018-12-12)
Alternative oxidase (AOX) is a non-mammalian enzyme that can bypass blockade of the complex III-IV segment of the respiratory chain (RC). We crossed a Ciona intestinalis AOX transgene into RC complex III (cIII)-deficient Bcs1l
Vincenzo Maione et al.
Biochimica et biophysica acta. General subjects, 1862(9), 1980-1987 (2018-05-31)
The CIA2A protein, in complex with CIAO1, has been proposed to be exclusively implicated in the maturation of cytosolic aconitase. However, how the CIA2A-CIAO1 complex generates active aconitase is still unknown and the available structural information has not provided any
Evaluation of the adaptogenic potential exerted by ginsenosides Rb1 and Rg1 against oxidative stress-mediated neurotoxicity in an in vitro neuronal model.
Carlos F M, et al.
PLoS ONE, 12(8), e0182933-e0182933 (2017)
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