重组
expressed in baculovirus infected Sf9 cells
质量水平
产品线
PRECISIO® Kinase
检测方案
≥70% (SDS-PAGE)
形式
buffered aqueous glycerol solution
比活
170-230 nmol/min·mg
分子量
~70 kDa
UniProt登记号
运输
dry ice
储存温度
−70°C
基因信息
human ... MYLK(4638)
生化/生理作用
MLCK 或肌球蛋白轻链激酶是免疫球蛋白基因超家族的肌肉成员，是一种钙/钙调蛋白依赖性蛋白激酶。MLCK 是一种平滑肌收缩调节蛋白，通过磷酸化 20-kDa 肌球蛋白轻链 (MLC20) 激活肌球蛋白腺苷三磷酸酶活性发挥作用。磷酸化的激酶活性位于 MLCK 的中心部分，其 N 端具有肌动蛋白结合活性，C 端具有肌球蛋白结合活性。肌球蛋白调节轻链的磷酸化在控制细胞分裂过程中的形态变化中起角色重要作用。
外形
50 mM Tris-HCl，pH 7.5，含 150 mM NaCl、0.2 5 mM DTT、0.1 mM EGTA、0.1 mM EDTA、0.1 mM PMSF 和 25% 甘油。
法律信息
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
F Matsumura et al.
Cell structure and function, 26(6), 639-644 (2002-04-11)
Phosphorylation of regulatory light chain (RMLC) of myosin II at Ser19/Thr18 is likely to play important roles in controlling the morphological changes seen during cell division of cultured mammalian cells. Phosphorylation of RMLC regulates the activity of myosin II, an
Kasturi Markandran et al.
International journal of molecular sciences, 23(1) (2022-01-12)
Heart failure (HF) as a result of myocardial infarction (MI) is a major cause of fatality worldwide. However, the cause of cardiac dysfunction succeeding MI has not been elucidated at a sarcomeric level. Thus, studying the alterations within the sarcomere
Y Gao et al.
IUBMB life, 51(6), 337-344 (2002-01-05)
Myosin light chain kinase (MLCK) is a regulatory protein for smooth muscle contraction, which acts by phosphorylating 20-kDa myosin light chain (MLC20) to activate the myosin ATPase activity. Although this mode of action is well-established, there are numerous reports of
Tünde Juhász et al.
FASEB bioAdvances, 2(8), 489-505 (2020-08-22)
Calmodulin (CaM), the key calcium sensor of eukaryotic cells regulating a great number of target proteins, belongs to the most conserved proteins. We compared function and properties of CaMs from two evolutionarily distant species, the human (Homo sapiens) representing vertebrates
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