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一般描述
氯化镁溶液适用于聚合酶链式反应(PCR)优化。氯化镁溶液提供聚合酶链式反应(PCR)所需镁离子。影响引物-模板退火温度、保真度、特异性和产量。过量镁会导致非特异性产物增加，镁缺乏会导致产量降低。
应用
氯化镁溶液已用作以下材料的组分：
- 聚合酶链式反应（PCR）扩增混合液
- 简单序列重复(ISSR)-PCR扩增混合液
- 16S-23S rRNA基因内转录间隔区（ITS）PCR扩增及限制性内切分析（ITS-PCR- rflp）反应缓冲液
- 用于定量逆转录-聚合酶链反应（qRT PCR）的PCR反应混合物，以扩增逆转录cDNA
- 用于RT-PCR检测基因表达的反应混合物
适用性
适用于聚合酶链反应的优化。
储存分类代码
12 - Non Combustible Liquids
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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实验方案
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