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一般描述
氯化镁溶液适用于聚合酶链式反应(PCR)优化。氯化镁溶液提供聚合酶链式反应(PCR)所需镁离子。影响引物-模板退火温度、保真度、特异性和产量。过量镁会导致非特异性产物增加,镁缺乏会导致产量降低。
应用
氯化镁溶液已用作以下材料的组分:
- 聚合酶链式反应(PCR)扩增混合液
- 简单序列重复(ISSR)-PCR扩增混合液
- 16S-23S rRNA基因内转录间隔区(ITS)PCR扩增及限制性内切分析(ITS-PCR- rflp)反应缓冲液
- 用于定量逆转录-聚合酶链反应(qRT PCR)的PCR反应混合物,以扩增逆转录cDNA
- 用于RT-PCR检测基因表达的反应混合物
适用性
适用于聚合酶链反应的优化。
储存分类代码
12 - Non Combustible Liquids
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
从最新的版本中选择一种:
Molecular characterization of Fusarium oxysporum f. sp. cubense isolates from banana
Pest Management in Horticultural Ecosystems , 18(2), 171-178 (2012)
American journal of perinatology, 36(10), 1060-1065 (2018-12-01)
Transcription of human endogenous retrovirus (HERV) elements is usually suppressed by epigenetic factors such as DNA methylation and heterochromatin silencing by histone modifications. There is an association between maternal smoking during pregnancy and DNA methylation levels in placental tissue and
Genome subtyping of autochthonous Bacillus species isolated from Iru, a fermented Parkia biglobosa seed
Food Biotechnology, 28(3), 250-268 (2014)
PloS one, 10(7), e0133841-e0133841 (2015-07-28)
The human WBSCR22 protein is a 18S rRNA methyltransferase involved in pre-rRNA processing and ribosome 40S subunit biogenesis. Recent studies have shown that the protein function in ribosome synthesis is independent of its enzymatic activity. In this work, we have
Scientific reports, 5, 12312-12312 (2015-07-24)
Inherited MYO5B mutations have recently been associated with microvillus inclusion disease (MVID), an autosomal recessive syndrome characterized by intractable, life-threatening, watery diarrhea appearing shortly after birth. Characterization of the molecular mechanisms underlying this disease and development of novel therapeutic approaches
商品
Quality DNA crucial for meaningful data; intact DNA key for successful chromosomal aberration analysis from FFPE tissues.
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实验方案
我们的SYBR Green qPCR实验方案是一种旨在准确定量基因表达和RT-PCR反应的的方法
Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions
使用CRISPR/Cas9系统生成正确的转基因小鼠需要充分理解正在测试的假设。了解如何创建成功的小鼠模型项目。
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