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Merck
CN

M8787

Sigma-Aldrich

氯化镁 溶液

PCR Reagent, 25 mM MgCI2 solution for PCR

别名:

PCR optimization

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About This Item

线性分子式:
MgCl2
CAS号:
分子量:
95.21
MDL编号:
UNSPSC代码:
12352302
PubChem化学物质编号:
NACRES:
NA.52
等级:
PCR Reagent
表单:
liquid

等级

PCR Reagent

质量水平

200
300

表单

liquid

包装

vial of 1.5 mL

浓度

25 mM±1 mM

技术

PCR: suitable

颜色

colorless

应用

agriculture
diagnostic assay manufacturing

异质活性

DNase, RNase, none detected

储存温度

2-8°C

SMILES字符串

Cl[Mg]Cl

InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2

InChI key

TWRXJAOTZQYOKJ-UHFFFAOYSA-L

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一般描述

氯化镁溶液适用于聚合酶链式反应(PCR)优化。氯化镁溶液提供聚合酶链式反应(PCR)所需镁离子。影响引物-模板退火温度、保真度、特异性和产量。过量镁会导致非特异性产物增加,镁缺乏会导致产量降低。

应用

氯化镁溶液已用作以下材料的组分:

  • 聚合酶链式反应(PCR)扩增混合液
  • 简单序列重复(ISSR)-PCR扩增混合液
  • 16S-23S rRNA基因内转录间隔区(ITS)PCR扩增及限制性内切分析(ITS-PCR- rflp)反应缓冲液
  • 用于定量逆转录-聚合酶链反应(qRT PCR)的PCR反应混合物,以扩增逆转录cDNA
  • 用于RT-PCR检测基因表达的反应混合物

适用性

适用于聚合酶链反应的优化。

储存分类代码

12 - Non Combustible Liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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访问文档库

Molecular characterization of Fusarium oxysporum f. sp. cubense isolates from banana
Das A, et al.
Pest Management in Horticultural Ecosystems , 18(2), 171-178 (2012)
Massimiliano Bergallo et al.
American journal of perinatology, 36(10), 1060-1065 (2018-12-01)
Transcription of human endogenous retrovirus (HERV) elements is usually suppressed by epigenetic factors such as DNA methylation and heterochromatin silencing by histone modifications. There is an association between maternal smoking during pregnancy and DNA methylation levels in placental tissue and
Genome subtyping of autochthonous Bacillus species isolated from Iru, a fermented Parkia biglobosa seed
Adewumi G A, et al.
Food Biotechnology, 28(3), 250-268 (2014)
Kadri Õunap et al.
PloS one, 10(7), e0133841-e0133841 (2015-07-28)
The human WBSCR22 protein is a 18S rRNA methyltransferase involved in pre-rRNA processing and ribosome 40S subunit biogenesis. Recent studies have shown that the protein function in ribosome synthesis is independent of its enzymatic activity. In this work, we have
Fernando Cartón-García et al.
Scientific reports, 5, 12312-12312 (2015-07-24)
Inherited MYO5B mutations have recently been associated with microvillus inclusion disease (MVID), an autosomal recessive syndrome characterized by intractable, life-threatening, watery diarrhea appearing shortly after birth. Characterization of the molecular mechanisms underlying this disease and development of novel therapeutic approaches

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