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产品名称
培养基 199, With Hanks′ salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
质量水平
无菌性
sterile-filtered
表单
liquid
技术
cell culture | mammalian: suitable
杂质
endotoxin, tested
组分
L-glutamine: no
NaHCO3: yes
Hanks’ salts (2% CO2): yes
sodium pyruvate: no
phenol red: yes
HEPES: no
运输
ambient
储存温度
2-8°C
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一般描述
原始培养基199的修改。Earles′盐被Hanks′盐代替。Hanks′盐使用较低浓度的碳酸氢盐和不同浓度和盐形式的必需无机离子。
培养基199是维生素、氨基酸和其他因素的组合，最初开发为用于原代外植体培养的完全确定的培养基配方。这种培养基添加适当的补充剂时具有广泛的适用性，特别是对于非转化细胞。培养基 199 广泛用于疫苗生产、原代胰腺外植体和晶状体组织的体外培养。
重悬
补充 0.1 g/L L-谷氨酰胺。
补充剂
通常也和此产品一起购买
产品编号
说明
价格
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
Michelle M Halstead et al.
Nature communications, 11(1), 4654-4654 (2020-09-19)
The shift from maternal to embryonic control is a critical developmental milestone in preimplantation development. Widespread transcriptomic and epigenetic remodeling facilitate this transition from terminally differentiated gametes to totipotent blastomeres, but the identity of transcription factors (TF) and genomic elements
Etienne de Coulon et al.
iScience, 24(2), 102041-102041 (2021-02-04)
A comprehensive understanding of mechano-electrical coupling requires continuous intracellular electrical recordings being performed on cells undergoing simultaneously in vivo like strain events. Here, we introduce a linear strain single-cell electrophysiology (LSSE) system that meets these requirements by delivering highly reproducible unidirectional
IGSF1 Does Not Regulate Spermatogenesis or Modify FSH Synthesis in Response to Inhibins or Activins.
Emilie Brûlé et al.
Journal of the Endocrine Society, 5(4), bvab023-bvab023 (2021-04-03)
Loss-of-function mutations in the X-linked immunoglobulin superfamily, member 1 (IGSF1) gene result in central hypothyroidism, often associated with macroorchidism. Testicular enlargement in these patients might be caused by increases in follicle-stimulating hormone (FSH) levels, as IGSF1 has been proposed to
Yining Li et al.
The Journal of biological chemistry, 292(6), 2301-2314 (2016-12-21)
Pituitary follicle-stimulating hormone (FSH) is an essential regulator of fertility in females and of quantitatively normal spermatogenesis in males. Pituitary-derived activins are thought to act as major stimulators of FSH synthesis by gonadotrope cells.
Benoît X E Desbiolles et al.
Microsystems & nanoengineering, 6, 67-67 (2021-09-28)
Volcano-shaped microelectrodes (nanovolcanoes) functionalized with nanopatterned self-assembled monolayers have recently been demonstrated to report cardiomyocyte action potentials after gaining spontaneous intracellular access. These nanovolcanoes exhibit recording characteristics similar to those of state-of-the-art micro-nanoelectrode arrays that use electroporation as an insertion
商品
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.
相关内容
Discover the formulation for Medium 199 for cell culture applications, including vaccine development.
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