Medium 199 Formulation

In 1950, J.F. Morgan conducted experiments with various combinations of vitamins, amino acids, and other factors to develop the novel culture media Medium 199. When properly supplemented, Medium 199 (M199) has broad species applicability, particularly for cultivation of non-transformed cells. It is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelial and rat lens tissues.