Home Mammalian Cell CultureDMEM/nutrient Mixture F-12 Formulation

DMEM/nutrient Mixture F-12 Formulation

DMEM/F12 medium is 1:1 mixture of Dulbecco's Modified Eagle's Medium (DMEM) and Ham's F-12 Nutrient Mixture. It is serum-free medium that contained insulin, transferrin, epidermal growth factor, leutinizing hormone or follicle stimulating hormone, somatomedin, and growth hormone first reported by Mather and Sato. DMEM/F12 media can also include HEPES buffer at a final concentration of 15 mM to compensate for the loss of buffering capacity from eliminating serum.

Click on the product numbers at the top of each column.

Component	D0547 g/L	D2906 g/L	D6421 [1x] g/L	D6434 [1x] g/L	D8062 [1x] g/L	D8437 [1x] g/L	D8900 g/L	D9785 g/L

Inorganic Salts
Ammonium Molybdate • 4H₂O	0.00000618	—	—	—	—	—	—	—

Ammonium Metavandate	0.00000058	—	—	—	—	—	—	—

Calcium Chloride	0.1166	0.1545	0.1166	0.1545	0.1166	0.1545	0.1545	—

Cupric Sulfate • 5H₂O	0.0000013	0.0000013	0.0000013	0.0000013	0.0000013	0.0000013	0.0000013	0.0000013

Ferric Nitrate • 9H₂O	0.00005	0.00005	0.00005	0.00005	0.00005	0.00005	0.00005	0.00005

Ferrous Sulfate • 7H₂O	0.000417	0.000417	0.000417	0.000417	0.000417	0.000417	0.000417	0.000417

Manganese Sulfate	0.000000151	—	—	—	—	—	—	—

Magnesium Chloride • 6H₂O	0.0612	0.0612	0.0612	0.0612	0.0612	0.0612	0.0612	—

Magnesium Sulfate (anhydrous)	0.04884	0.04884	0.04884	0.04884	0.04884	0.04884	0.04884	—

Nickel Chloride	0.00000012	—	—	—	—	—	—	—

Potassium Chloride	0.3118	0.3118	0.3118	0.3118	0.3118	0.3118	0.3118	0.3118

Sodium Bicarbonate	—	—	1.2	—	—	1.2	—	—

Sodium Chloride	6.996	6.996	6.996	6.996	6.996	6.996	6.996	6.996

Sodium Metasilicate • 9H₂O	0.0000142	—	—	—	—	—	—	—

Sodium Selenite	0.00000519	—	—	—	—	—	—	—

Sodium Phosphate Dibasic (anhydrous)	0.07102	0.07102	0.07102	0.07102	0.07102	0.07102	0.07102	0.07102

Sodium Phosphate Monobasic (anhydrous)	0.0543	0.0543	0.0543	0.0543	0.0543	0.0543	0.0543	0.0543

Stannous Chloride • 2H₂O	0.00000011	—	—	—	—	—	—	—

Zinc Sulfate • 7H₂O	0.000432	0.000432	0.000432	0.000432	0.000432	0.000432	0.000432	0.000432

Amino Acids
L-Alanine	0.00445	0.00445	0.00445	0.00445	0.00445	0.00445	0.00445	0.00445

L-Arginine • HCl	0.1475	0.1475	0.1475	0.1475	0.1475	0.1475	0.1475	0.1475

L-Asparagine • H₂O	0.0075	0.0075	0.0075	0.0075	0.0075	0.0075	0.0075	0.0075

L-Aspartic Acid	0.00665	0.00665	0.00665	0.00665	0.00665	0.00665	0.00665	0.00665

L-Cystine • 2HCl	0.03129	0.03129	0.03129	0.03129	0.03129	0.03129	0.03129	0.03129

L-Cysteine • HCl • H₂O	0.01756	0.01756	0.01756	0.01756	0.01756	0.01756	0.01756	0.01756

L-Glutamic Acid	0.00735	0.00735	0.00735	0.00735	0.00735	0.00735	0.00735	0.00735

L-Glutamine	0.365	0.365	—	—	0.365	0.365	0.365	—

Glycine	0.01875	0.01875	0.01875	0.01875	0.01875	0.01875	0.01875	0.01875

L-Histidine • HCl • H₂O	0.03148	0.03148	0.03148	0.03148	0.03148	0.03148	0.03148	0.03148

L-Isoleucine	0.05447	0.05447	0.05447	0.05447	0.05447	0.05447	0.05447	0.05447

L-Leucine	0.05905	0.05905	0.05905	0.05905	0.05905	0.05905	0.05905	—

L-Lysine • HCl	0.09125	0.09125	0.09125	0.09125	0.09125	0.09125	0.09125	—

L-Methionine	0.01724	0.01724	0.01724	0.01724	0.01724	0.01724	0.01724	—

L-Phenylalanine	0.03548	0.03548	0.03548	0.03548	0.03548	0.03548	0.03458	0.03548

L-Proline	0.01725	0.01725	0.01725	0.01725	0.01725	0.01725	0.01725	0.01725

L-Serine	0.02625	0.02625	0.02625	0.02625	0.02625	0.02625	0.02625	0.02625

L-Threonine	0.05345	0.05345	0.05345	0.05345	0.05345	0.05345	0.05345	0.05345

L-Tryptophan	0.00902	0.00902	0.00902	0.00902	0.00902	0.00902	0.00902	0.00902

L-Tyrosine • 2Na • 2H₂O	0.05579	0.05579	0.05579	0.05579	0.05579	0.05579	0.05579	0.05579

L-Valine	0.05285	0.05285	0.05285	0.05285	0.05285	0.05285	0.05285	0.05285

Vitamins
D-Biotin	0.0000035	0.0000035	0.0000035	0.0000035	0.0000035	0.0000035	0.0000035	0.0000035

Choline Chloride	0.00898	0.00898	0.00898	0.00898	0.00898	0.00898	0.00898	0.00898

Folic Acid	0.00265	0.00265	0.00265	0.00266	0.00266	0.00266	0.00266	0.00266

myo-Inositol	0.0126	0.0126	0.0126	0.0126	0.0126	0.0126	0.0126	0.0126

Niacinamide	0.00202	0.00202	0.00202	0.00202	0.00202	0.00202	0.00202	0.00202

D-Pantothenic Acid (hemicalcium)	0.00224	0.00224	0.00224	0.00224	0.00224	0.00224	0.00224	0.00224

Pyridoxal • HCl	0.002	0.002	—	0.002	0.002	0.002	0.002	0.002

Pyridoxine • HCl	0.002031	0.002031	0.002031	—	—	0.000031	0.000031	0.000031

Riboflavin	0.000219	0.000219	0.000219	0.000219	0.000219	0.000219	0.000219	0.000219

Thiamine • HCl	0.00217	0.00217	0.00217	0.00217	0.00217	0.00217	0.00217	0.00217

Vitamin B12	0.00068	0.00068	0.00068	0.00068	0.00068	0.00068	0.00068	0.00068

Other
D-Glucose	3.15	3.15	3.15	3.15	3.15	3.15	3.15	3.15

HEPES	—	3.5745	3.5745	3.5745	—	3.5745	3.5745	3.5745

Hypoxanthine	0.0021	0.00244	0.0021	0.00244	0.0021	0.00244	0.00244	0.0021

Linoleic Acid	0.000042	0.000042	0.000042	0.000042	0.000042	0.000042	0.000042	0.000042

Phenol Red • Na	0.00863	—	0.00863	—	0.00863	0.00863	0.00863	—

Putrescine • HCl	0.000081	0.000081	0.000081	0.000081	0.000081	0.000081	0.000081	0.000081

Pyruvic Acid • Na	0.11	0.055	0.055	0.055	0.055	0.055	0.055	0.055

DL-Thioctic Acid	0.000105	0.000105	0.000105	0.000105	0.000105	0.000105	0.000105	0.000105

Thymidine	0.000365	0.000365	0.000365	0.000365	0.000365	0.000365	0.000365	0.000365

Add
Sodium Bicarbonate	1.2	1.2	—	—	—	—	1.2	1.2

Calcium Chloride • 2H₂O	—	—	—	—	—	—	—	0.1545

L-Glutamine	—	—	0.365	0.365	—	—	—	0.365

L-Leucine	—	—	—	—	—	—	—	0.05905

L-Lysine • HCl	—	—	—	—	—	—	—	0.09125

Magnesium Chloride • 6H₂O	—	—	—	—	—	—	—	0.0612

Magnesium Sulfate	—	—	—	—	—	—	—	0.04884

L-Methionine	—	—	—	—	—	—	—	0.0172