D9785
DMEM/F-12
With HEPES, without ʟ-glutamine, ʟ-leucine, ʟ-lysine, ʟ-methionine, CaCl2, MgCl₂, MgSO4, sodium bicarbonate, and phenol red, powde, suitable for cell cutlure
Synonym(s):
Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham, DME/F-12, 1:1 mixture
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About This Item
Product Name
DMEM/Nutrient Mixture F-12 Ham, With 15 mM HEPES, without
form
powder
technique(s)
cell culture | mammalian: suitable
components
L-glutamine: no
HEPES: 15 mM
sodium pyruvate: 0.055 g/L
NaHCO3: no
phenol red: no
glucose: 3.15 g/L
shipped in
ambient
storage temp.
2-8°C
General description
Listen to an Audio Overview
The most basic formulation offered. This formulation is used by investigators who want to start with the essential components of (1;1) DME/Ham′s F-12, and have the flexibility to optimize the formula for their own application. This base formulation is particularly suitable for studies of cell protein metabolism with labeL -tagged amino acids.
The most basic formulation offered. This formulation is used by investigators who want to start with the essential components of (1;1) DME/Ham′s F-12, and have the flexibility to optimize the formula for their own application. This base formulation is particularly suitable for studies of cell protein metabolism with labe
Preparation Note
Formulated to contain 14.8 grams of powder per liter of medium.
Supplement with 0.1545 g/L calcium chloride, 0.365 g/L L -glutamine, 0.05905 g/L L -leucine, 0.09125 g/L L -lysine, 0.0612 g/L magnesium chloride, 0.04884 g/L magnesium sulfate, 0.0172 g/L L -methionine, 1.2 g/L sodium bicarbonate.
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Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
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