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Merck
CN

M5899

Sigma-Aldrich

抗-小鼠 IgG(全分子) 山羊抗

whole antiserum

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MDL编号:
UNSPSC代码:
12352203
NACRES:
NA.46

生物来源

goat

质量水平

偶联物

unconjugated

抗体形式

whole antiserum

抗体产品类型

secondary antibodies

克隆

polyclonal

包含

15 mM sodium azide

技术

indirect ELISA: 1:70,000
quantitative precipitin assay: 3.2-4.8 mg/mL

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

unmodified

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相关类别

一般描述

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.

特异性

The antiserum is determined to be immunospecific for mouse IgG by immunoelectrophoresis (IEP) against normal mouse serum and purified mouse IgG.

应用

Anti-Mouse IgG (whole molecule) antibody produced in goat has been used as secondary antibody in:
  • immunoblotting
  • immunohistochemistry
  • proteomic microarrays
Anti-Mouse IgG (whole molecule) antibody produced in goat has been used in:
  • the preparation of immunochromatography strips for magneto-enzyme lateral flow immunoassay
  • immunochromatography
  • MYCN DNA-binding analysis (ChIP-seq)

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)

生化/生理作用

IgG antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.
Immunoglobulin G (IgG) helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

质量

The antiserum has been treated to remove lipoproteins.

外形

Supplied as a liquid containing 15 mM sodium azide as preservative.

储存及稳定性

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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David J Duffy et al.
Oncotarget, 7(37), 60310-60331 (2016-08-18)
Wnt signalling is involved in the formation, metastasis and relapse of a wide array of cancers. However, there is ongoing debate as to whether activation or inhibition of the pathway holds the most promise as a therapeutic treatment for cancer
Tien V Tran et al.
PeerJ, 7, e7779-e7779 (2019-10-04)
Dengue infection represents a global health issue of growing importance. Dengue non-structural protein 1 (NS1) plays a central role in the early detection of the disease. The most common method for NS1 detection is testing by lateral flow immunoassays (LFIAs)
Katja C Erlach et al.
Journal of virology, 76(6), 2857-2870 (2002-02-28)
Tumor relapse and cytomegalovirus (CMV) infection are major concerns in the therapy of hematopoietic malignancies by bone marrow transplantation (BMT). Little attention so far has been given to a possible pathogenetic interplay between CMV and lymphomas. CMV inhibits stem cell
Natascha Krömmelbein et al.
Viruses, 8(2) (2016-02-06)
The human cytomegalovirus (HCMV) replicates to high titers in primary human fibroblast cell cultures. A variety of primary human cells and some tumor-derived cell lines do also support permissive HCMV replication, yet at low levels. Cell lines established by transfection
Barbara Wróblewska et al.
Nutrients, 14(20) (2022-10-28)
Barley (Hordeum vulgare L.) proteins are taxonomically homologous to wheat proteins and react with sera from patients with baker's asthma. In the current work, the crude extract of barley proteins was divided into six fractions on DEAE-Sepharose. Their immunoreactivity in

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