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Merck
CN

M4159

Sigma-Aldrich

亚甲蓝 水合物

suitable for nucleic acid staining, BioReagent

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别名:
3,7-双(二甲基氨基)吩噻嗪-5-鎓氯化物, 亚甲蓝, 亚甲蓝一水合物, 核酸染色试剂
经验公式(希尔记法):
C16H18ClN3S · xH2O
分子量:
319.85 (anhydrous basis)
Beilstein:
3599847
EC 号:
MDL编号:
UNSPSC代码:
12352200
PubChem化学物质编号:
NACRES:
NA.52

等级

for molecular biology

质量水平

产品线

BioReagent

形式

powder

技术

nucleic acid detection: suitable

溶解性

water: 1 mg/mL (dark blue)

适用性

suitable for nucleic acid staining

储存温度

room temp

SMILES字符串

O.[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

InChI

1S/C16H18N3S.ClH.H2O/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;;/h5-10H,1-4H3;1H;1H2/q+1;;/p-1

InChI key

WQVSELLRAGBDLX-UHFFFAOYSA-M

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相关类别

一般描述

亚甲蓝是一种杂环芳香化学品,呈深绿色固体粉末状,在水溶液中会变为蓝色。它在许多不同的组织学过程以及分子生物学实验室中被用作染色剂。

应用

亚甲基蓝水合物适合用作琼脂糖和聚丙烯酰胺凝胶电泳(PAGE)的核酸染液。通过使用克隆形成试验,被用于对4T1肿瘤细胞进行染色以研究肿瘤细胞的转移。亚甲基蓝水合物晶体通过被包封在脱铁蛋白纳米笼中,可用于抗肿瘤细胞的光动力学疗法。

特点和优势

  • 比溴化乙锭染色的危害更小
  • 可在可见光下观察
  • 快速且易于使用。

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Acute Tox. 4 Oral

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves


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Angela L Davis et al.
International journal of molecular sciences, 14(2), 4185-4202 (2013-02-23)
Repurposing approved and abandoned non-oncological drugs is an alternative developmental strategy for the identification of anticancer therapeutics that has recently attracted considerable attention. Due to the essential role of the cellular heat shock response in cytoprotection through the maintenance of
Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis.
A C Peacock et al.
Biochemistry, 6(6), 1818-1827 (1967-06-01)
Young-Sharp, D. and Kumar, R.
Technique, 1, 183-183 (1989)
Domenic Di Paola et al.
Nucleic acids research, 38(7), 2314-2331 (2010-01-13)
Using libraries of replication origins generated previously, we identified three clones that supported the autonomous replication of their respective plasmids in transformed, but not in normal cells. Assessment of their in vivo replication activity by in situ chromosomal DNA replication

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