X4126
二甲苯青FF
BioReagent, Molecular Biology
别名:
XC, 二甲苯青FF, 酸性蓝147
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关于此项目
经验公式（希尔记法）:
C25H27N2NaO6S2
化学文摘社编号:
分子量:
538.61
UNSPSC Code:
12171500
NACRES:
NA.52
PubChem Substance ID:
EC Number:
220-167-5
Colour Index Number:
42135
MDL number:
InChI key
VVLFAAMTGMGYBS-KRQUPCAFSA-M
InChI
1S/C25H28N2O6S2.Na/c1-5-26-22-11-7-18(13-16(22)3)25(19-8-12-23(27-6-2)17(4)14-19)21-10-9-20(34(28,29)30)15-24(21)35(31,32)33;/h7-15,26H,5-6H2,1-4H3,(H,28,29,30)(H,31,32,33);/q;+1/p-1/b25-19-,27-23-;
SMILES string
[Na+].CCNc1ccc(cc1C)C(=C2/C=CC(=N\CC)\C(C)=C2)\c3ccc(cc3S(O)(=O)=O)S([O-])(=O)=O
grade
Molecular Biology
product line
BioReagent
form
powder
technique(s)
nucleic acid detection: suitable
solubility
H2O: complete 1 mg/mL, clear, blue
suitability
suitable for nucleic acid staining
storage temp.
room temp
Quality Level
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Application
二甲苯青 FF用作：
- 组织染色、DNA测序和电泳的示踪染料
- 凝胶上样染料
- 甲酰胺RNA上样染料
- 天然聚丙烯酰胺凝胶电泳（PAGE）上样缓冲液的示踪染料
Biochem/physiol Actions
二甲苯青FF可充当核酸染色剂。
General description
在琼脂糖或聚丙烯酰胺凝胶电泳期间，二甲苯蓝通常用作跟踪染料。 它具有轻微的负电荷并且可与DNA相同的方向进行迁移，允许用户对分子在凝胶中移动的进程进行监测。 迁移速率随凝胶组成而变化。
Preparation Note
可以使用标准配方制备6x凝胶上样缓冲液，其中包含0.05％二甲苯蓝。
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
Single nucleotide polymorphism detection by polymerase chain reaction-restriction fragment length polymorphism
<BIG>Ota M, et al.</BIG>
Nature Protocols, 2857-2857 (2007)
Nicolás Cara et al.
Bio-protocol, 10(13), e3671-e3671 (2021-03-05)
Methylation-Sensitive Amplification Polymorphism (MSAP) is a versatile marker for analyzing DNA methylation patterns in non-model species. The implementation of this technique does not require a reference genome and makes it possible to determine the methylation status of hundreds of anonymous
P N Hengen
Trends in biochemical sciences, 20(5), 202-203 (1995-05-01)
Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methods-reagents, available on the Internet. This month's column describes how to make an electrophoresis system from common household items. For details on how to partake
Design and self-assembly of siRNA-functionalized RNA nanoparticles for use in automated nanomedicine
<BIG>Afonin KA, et al.</BIG>
Nature Protocols, 6, 2022-2022 (2011)
Cell-internalization SELEX: method for identifying cell-internalizing RNA aptamers for delivering siRNAs to target cells
<BIG>Thiel WH, et al.</BIG>
Methods in Molecular Biology, 187-199 (2015)
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