Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
L-glutamine: no
phenol red: no
HEPES: no
sodium pyruvate: no
Earle’s salts (5% CO2): yes
NaHCO3: no
shipped in
ambient
storage temp.
2-8°C
General description
培养基199是维生素、氨基酸和其他因素的组合，最初开发为用于原代外植体培养的完全确定的培养基配方。这种培养基添加适当的补充剂时具有广泛的适用性，特别是对于非转化细胞。培养基 199 广泛用于疫苗生产、原代胰腺外植体和晶状体组织的体外培养。
Application
培养基199已用于：
- 培养原代人脐静脉内皮细胞（HUVEC），以用于细胞活力检测
- 冲洗小母猪子宫角，从子宫恢复第12天妊娠
- 培养腹膜细胞和永生化人间皮细胞（MeT-5A和ATCC-CRL-9444）
Preparation Note
补充0.1克/升左旋谷氨酰胺、2.2克/升碳酸氢钠。
配制成每升培养基中含 9.4 克粉末。
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存储类别
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
实验方案
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性，应注意防潮。
商品
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.
Luciana de Lima Ferreira et al.
PLoS neglected tropical diseases, 14(12), e0009015-e0009015 (2020-12-29)
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Diane G Edmondson et al.
mBio, 12(1) (2021-02-25)
The bacterium that causes syphilis, Treponema pallidum subsp. pallidum, has now been cultured in vitro continuously for periods exceeding 3 years using a system consisting of coculture with Sf1Ep rabbit epithelial cells in TpCM-2 medium and a low-oxygen environment. In
Hung-Chang Chou et al.
Colloids and surfaces. B, Biointerfaces, 208, 112136-112136 (2021-10-11)
Nitric oxide (NO) is a potential therapeutic agent for various diseases. However, it is challenging to deliver this unstable, free-radical gaseous molecule in the body. Various nanoparticle-based drug delivery systems have been investigated as promising NO carriers without detailed characterization
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| M3769-50L | 04061834050568 |
| M3769-10X1L | 04061834050551 |