product name
4-甲基伞形酮磷酸酯 (4-MUP) 液体底物系统, liquid
形式
liquid
质量水平
储存温度
2-8°C
SMILES字符串
CC1=CC(=O)Oc2cc(OP(O)(O)=O)ccc12
InChI
1S/C10H9O6P/c1-6-4-10(11)15-9-5-7(2-3-8(6)9)16-17(12,13)14/h2-5H,1H3,(H2,12,13,14)
InChI key
BCHIXGBGRHLSBE-UHFFFAOYSA-N
相关类别
一般描述
4-甲基伞形酮磷酸酯 (4-MUP) 是碱性磷酸酶的底物，碱性磷酸酶形成可溶性荧光物质甲基伞形酮。4-MUP 液体底物系统在即用型试剂中结合了 4-MUP 和缓冲液。
应用
4-甲基伞形磷酸酯（4-MUP）液体底物系统已被用作酶联免疫吸附测定（ELISA）中碱性磷酸盐的荧光底物。它也已被用于分泌的胚胎碱性磷酸酶（SEAP）活性测定中。
分析说明
4-MUP 荧光可在 360 nm 激发和 440 nm 发射下测量。也可使用紫外光源观察荧光终产物。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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As part of a program to develop better PTP1B fluorogenic substrates that more closely mimic the functionality found in the natural substrate, we have prepared and evaluated nine novel analogs of 4-methylumbelliferone phosphate (MUP) with a variety of additional groups
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