质量水平
形式
powder
技术
cell culture | mammalian: suitable
组分
Earle’s salts (5% CO2): yes
L-glutamine: no
HEPES: no
NaHCO3: no
sodium pyruvate: no
phenol red: no
运输
ambient
储存温度
2-8°C
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一般描述
Eagle最低必需培养基含有19种氨基酸。包括必需氨基酸和非必需氨基酸：丙氨酸(L-ala)、天冬酰胺(L-asn)、天冬氨酸(L-asp)、谷氨酸(L-glu)、甘氨酸(L-gly)、脯氨酸(L-pro)和丝氨酸(L-ser)。可优先用于在减少的血清存在下的贴壁细胞的生长或作为开发无血清培养基的基础配方。适合多种细胞的单层培养。
应用
Eagle最低必需培养基已用于：
- 培养Ishikawa子宮內膜腺癌细胞，用于双酚研究
- 培养MCF-7细胞，用于激素处理实验
- 维持斑马鱼松果体活性，用于作用光谱分析
特点和优势
含有高浓度的氨基酸，因此培养基成分类似于哺乳动物培养细胞的蛋白质成分。
数量
配制成每升培养基含 9.3 克粉末。
重悬
补充 0.292 g/L左旋谷氨酰胺、2.2 g/L 碳酸氢钠。
补充剂
通常也和此产品一起购买
产品编号
说明
价格
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
Anatoli Y Kabakov et al.
PloS one, 10(4), e0123718-e0123718 (2015-04-24)
During long-term potentiation (LTP) of excitatory synapses, Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) is activated by Ca(2+) influx through NMDA receptors that potentiate AMPA receptor currents by insertion of additional GluR1-containing receptors at the synapse and by increasing AMPA channel conductance
Kyle S Enger et al.
Journal of water and health, 14(6), 950-960 (2016-12-14)
Many different household water treatment (HWT) methods have been researched and promoted to mitigate the serious burden of diarrheal disease in developing countries. However, HWT methods using bromine have not been extensively evaluated. Two gravity-fed HWT devices (AquaSure™ and Waterbird™)
Hongjie Fan et al.
Cells, 10(11) (2021-11-28)
Bisphenol A (BPA) is a well-known endocrine disruptor, widely used in various consumer products and ubiquitously found in air, water, food, dust, and sewage leachates. Recently, several countries have restricted the use of BPA and replaced them with bisphenol S
Congcong Wang et al.
Ecotoxicology and environmental safety, 207, 111299-111299 (2020-09-15)
The analogues of biphenol A (BPA), including bisphenol S (BPS) and bisphenol B (BPB), are commonly used to replace the application of BPA in containers and wrappers of daily life. However, their safeties are questioned due to their similar chemical
Tiefeng Cao et al.
Oncogene, 38(27), 5356-5366 (2019-05-16)
Uterine leiomyomas or fibroids (UFs) are benign tumors characterized by hyperplastic smooth muscle cells and excessive deposition of extracellular matrix (ECM). Afflicting ~80% of women, and symptomatic in 25%, UFs bring tremendous suffering and are an economic burden worldwide; they
商品
A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
实验方案
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性，应注意防潮。
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