质量水平
形式
powder
技术
cell culture | mammalian: suitable
组分
L-glutamine: yes
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: no
sodium pyruvate: no
HEPES: 25 mM
运输
ambient
储存温度
2-8°C
正在寻找类似产品? 访问 产品对比指南
一般描述
培养基199是维生素、氨基酸和其他因素的组合，最初开发为用于原代外植体培养的完全确定的培养基配方。这种培养基添加适当的补充剂时具有广泛的适用性，特别是对于非转化细胞。培养基 199 广泛用于疫苗生产、原代胰腺外植体和晶状体组织的体外培养。
应用
培养基199已用于：
- 在成熟前保持卵丘-卵母细胞复合体（COC），并培养这些卵母细胞在体外成熟
- 在德国荷斯坦奶牛卵子采集过程中清洗抽吸管
- 培养大鼠心脏内皮细胞
数量
配制成每升培养基含 14.7 克粉末。
重悬
补充 2.2 g/L 碳酸氢钠。
补充剂
通常也和此产品一起购买
产品编号
说明
价格
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
In vitro nuclear maturation of bitch oocytes in the presence of polyvinyl-pyrrolidone
Animal Reproduction, 3(1), 70-75 (2018)
Mao Ye et al.
Scientific reports, 4, 4681-4681 (2014-04-16)
The highly specialized endothelial cells in brain capillaries are a key component of the blood-brain barrier, forming a network of tight junctions that almost completely block paracellular transport. In contrast to vascular endothelial cells in other organs, we show that
Jarosław Wieczorek et al.
Animals : an open access journal from MDPI, 10(4) (2020-04-16)
The laparoscopic method of recovering oocytes in goats and sheep is one of the minimally invasive methods used in the biotechnology of animal reproduction. It allows for good quality oocytes that are suitable for in vitro maturation and fertilization to
L González-Fernández et al.
Reproduction, fertility, and development, 30(2), 297-306 (2017-07-07)
Equine cumulus-oocyte complexes (COCs) are classified as compact (cCOC) or expanded (eCOC) and vary in their meiotic competence. This difference could be related to divergent glucose metabolism. To test this hypothesis in the present study, eCOCs, cCOCs and expanded or
A J Korzekwa et al.
Polish journal of veterinary sciences, 19(3), 609-617 (2016-10-21)
Adenomyosis is defined as the presence of glandular foci external to the endometrium of the uterus, either in the myometrium or/and perimetrium, depending on the progress of this dysfunction. To date, we showed that steroids secretion and prolactin expression and
商品
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.
Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.
钙是一种离子稳定的二价阳离子，在细胞培养基中具有有益和有毒的双重属性，可作为一系列细胞培养基的组成部分。
实验方案
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性，应注意防潮。
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门