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M2003

Sigma-Aldrich

MTT 甲臜

powder

别名:

1-（4，5-二甲基吡啶-2-基）-3，5-二苯基甲酰胺, 噻唑基蓝甲臜

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About This Item

经验公式（希尔记法）:
C18H17N5S
CAS号:
57360-69-7
分子量:
335.43
MDL编号:
MFCD00080731
UNSPSC代码:
12171500
PubChem化学物质编号:
24896591
NACRES:
NA.47

方案

≥97% (TLC)

质量水平

200

表单

powder

颜色

purple

溶解性

acetone: 1 mg/mL

应用

diagnostic assay manufacturing
hematology
histology

储存温度

room temp

SMILES字符串

Cc1nc(\N=N\C(=N\Nc2ccccc2)c3ccccc3)sc1C

InChI

1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+

InChI key

GIFKVQOPAJDJLO-SZVLODPRSA-N

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相关类别

应用

MTT 甲臜已被用于测量细胞活力。

生化/生理作用

MTT法是一种比色反应分析方法，常用于检测细胞活率。该方法要求将溶于水的黄色3-[4,5-二甲基噻唑-2-yl]-2,5-基四氮唑嗅盐(MTT)酶促还原为不溶于水的紫色MTT-甲臜。该反应由线粒体脱氢酶催化，仅在活细胞中发生。因此，蓝色甲臜量与活细胞数量成正比。通过分光光度法分析570 nm波长处的甲臜溶液吸光度。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

从最新的版本中选择一种：

分析证书(COA)

Lot/Batch Number
0000325975
0000325975
0000210043
0000210043
SHBQ7917

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Can MTT Formazan, Product M2003, be used in cell viability assays?

    No.  MTT or thiazolyl blue tetrazolium is used to determine the number of viable cells in well-cultures. Consider use of kit CGD1 or product M5655.

  4. What is the solubility of MTT Formazan, Product M2003, in water or PBS?

    This product is not soluble in either water or PBS.  Sigma Aldrich tests product at 1mg/mL in acetone. A 1:1 DMSO:acidified isopropanol also works well for dissolving the formazan.

  5. Can MTT Formazan, Product M2003, be used to measure dehydrogenases?

    No. MTT is converted by active mitochondrial dehydrogenases of living cells to MTT Formazan. Consider use of product M2128 or M5655.

  6. What is the proper wavelength for measuring MTT Formazan, Product M2003?

    We recommend detection at 570 nm, with background subtraction at 630-690 nm in acidified isopropanol.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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