product name
Eagle最低必需培养基, With Hanks′ salts, L-glutamine and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture
形式
powder
技术
cell culture | mammalian: suitable
组分
phenol red: yes
NaHCO3: no
HEPES: no
sodium pyruvate: no
Hanks’ salts (2% CO2): yes
L-glutamine: yes
运输
ambient
储存温度
2-8°C
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一般描述
它是经典MEM配方的改良版。厄尔′盐类已被汉克′盐类所取代。由于碳酸氢盐浓度显著降低，因此该介质具有显著降低的缓冲能力。含有19个氨基酸。必需氨基酸和非必需氨基酸；L-ala；L-asn；L-asp；L-glu；L-gly；L-pro和L-ser。可优先用于在减少的血清存在下的贴壁细胞的生长或作为开发无血清培养基的基础。
数量
按照每升培养基10.7克粉末配制。
重悬
补充0.35 g/L碳酸氢钠。
补充剂
通常也和此产品一起购买
产品编号
说明
价格
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
Claudia Penna et al.
Cellular and molecular neurobiology, 30(8), 1171-1179 (2010-11-26)
The Chromogranin A (CgA)-derived anti-hypertensive peptide catestatin (CST) antagonizes catecholamine secretion, and is a negative myocardial inotrope acting via a nitric oxide-dependent mechanism. It is not known whether CST contributes to ischemia/reperfusion injury or is a component of a cardioprotective
Tadashi Yoshida et al.
Cellular signalling, 73, 109690-109690 (2020-06-20)
Minocycline, an FDA-approved second-generation semisynthetic tetracycline, exerts antioxidant, anti-apoptotic and anti-inflammatory effects, independent of its antimicrobial properties. Interleukin (IL)-17A is an immune and inflammatory mediator, and its sustained induction is associated with various cardiovascular diseases. Here we investigated (i) whether
Melina J Sedano et al.
Cancers, 12(6) (2020-06-14)
Hypoxanthine phosphoribosyl transferase 1 (HPRT1) is traditionally believed to be a housekeeping gene; however, recent reports suggest that it is upregulated in several cancers and is associated with clinical outcomes. HPRT1 is located on chromosome X and encodes the HPRT
Nguyen T K Vo et al.
Developmental and comparative immunology, 92, 140-149 (2018-11-20)
Viral double-stranded (ds)RNA is a potent pathogen-associated molecular pattern (PAMP), capable of inducing a strong antiviral state within the cell, protecting the cell from virus infection. In mammals and fish, sensing extracellular dsRNA is mediated by cell-surface class A scavenger
Mario G Pavez-Giani et al.
International journal of molecular sciences, 22(9) (2021-05-01)
ATPase inhibitory factor-1 (IF1) preserves cellular ATP under conditions of respiratory collapse, yet the function of IF1 under normal respiring conditions is unresolved. We tested the hypothesis that IF1 promotes mitochondrial dysfunction and pathological cardiomyocyte hypertrophy in the context of
商品
A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
实验方案
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性，应注意防潮。
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