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质量水平
表单
powder
技术
cell culture | mammalian: suitable
组分
NaHCO3: no
phenol red: yes
Earle’s salts (5% CO2): yes
L-glutamine: no
sodium pyruvate: no
HEPES: no
运输
ambient
储存温度
2-8°C
一般描述
它是经典MEM配方的改良版。含有琥珀酸盐缓冲系统。有关具体信息,请参阅配方表。培养基可以高压灭菌。
其他说明
经改良适用于高压灭菌。
数量
按照每升培养基9.4克粉末配制。
重悬
补充0.292 g/L L-谷氨酰胺,2.2 g/L碳酸氢钠。
法律信息
Auto-Mod is a trademark of Sigma-Aldrich Co. LLC
补充剂
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Simona Giunta et al.
The Journal of cell biology, 190(2), 197-207 (2010-07-28)
The signaling cascade initiated in response to DNA double-strand breaks (DSBs) has been extensively investigated in interphase cells. Here, we show that mitotic cells treated with DSB-inducing agents activate a "primary" DNA damage response (DDR) comprised of early signaling events
Pasqualino de Antonellis et al.
PloS one, 6(9), e24584-e24584 (2011-09-21)
Through negative regulation of gene expression, microRNAs (miRNAs) can function as oncosuppressors in cancers, and can themselves show altered expression in various tumor types. Here, we have investigated medulloblastoma tumors (MBs), which arise from an early impairment of developmental processes
Carlos Mendoza-Palomares et al.
Eukaryotic cell, 7(4), 684-697 (2008-02-19)
Cysteine proteases have been shown to be essential virulence factors and drug targets in trypanosomatids and an attractive antidisease vaccine candidate for Trypanosoma congolense. Here, we describe an important amplification of genes encoding cathepsin B-like proteases unique to T. congolense.
Hisashi Miura et al.
Nucleic acids research, 37(3), 778-792 (2008-12-17)
Mammalian tissue- and/or time-specific transcription is primarily regulated in a combinatorial fashion through interactions between a specific set of transcriptional regulatory factors (TRFs) and their cognate cis-regulatory elements located in the regulatory regions. In exploring the DNA regions and TRFs
Michelle Griffin et al.
PloS one, 6(8), e23404-e23404 (2011-08-23)
Non-unions pose complications in fracture management that can be treated using electrical stimulation (ES). Bone marrow mesenchymal stem cells (BMMSCs) are essential in fracture healing; however, the effect of different clinical ES waveforms on BMMSCs cellular activities remains unknown. We
商品
A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
实验方案
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性,应注意防潮。
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