质量水平
形式
powder
技术
cell culture | mammalian: suitable
组分
NaHCO3: no
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: yes
运输
ambient
储存温度
2-8°C
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一般描述
最低必需培养基(MEM)是Harry Eagle开发的合成细胞培养基。培养基含有较高浓度的氨基酸，因此比较接近哺乳动物培养细胞的蛋白质成分。在含有Hanks′或Earle′s盐的配方中选择补充非必需氨基酸，可扩大该培养基的用途。混合Earle′s平衡盐的MEM是改良配方，又名αMEM，包含非必需氨基酸、丙酮酸钠和额外的维生素。
应用
Eagle最低必需培养基已用于：
- 自然杀伤细胞(NK)的维持培养
- 杜氏利什曼原虫(Leishmania donovani)菌株培养
- 大鼠毛囊隆突部位表皮神经嵴干细胞(EPI-NCSCs)的培养
数量
按照每升培养基10.1克粉末配制。
重悬
补充2.2 g/L碳酸氢钠。
补充剂
通常也和此产品一起购买
产品编号
说明
价格
警示用语：
Warning
危险声明
危险分类
Skin Sens. 1
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
C C Lachaud et al.
Cell death & disease, 5, e1304-e1304 (2014-06-27)
Recent studies suggested that the post-natal mesothelium retain differentiative potential of the embryonic mesothelium, which generates fibroblasts and vascular smooth muscle cells (VSMCs), in developing coelomic organs via epithelial-to-mesenchymal transition (EMT). Whether adult mesothelial cells (MCs) are able to give
Hannah Finke et al.
Journal of orofacial orthopedics = Fortschritte der Kieferorthopadie : Organ/official journal Deutsche Gesellschaft fur Kieferorthopadie, 79(5), 328-336 (2018-07-18)
Miniscrews are an important choice for orthodontic anchorage. Yet reports on failures do exist, and attempts have been made to elucidate the causes. Clinical outcomes may be compromised not only by the mechanical implications of miniscrew design and the location
Kyoko Fujisawa et al.
PLoS neglected tropical diseases, 15(7), e0009552-e0009552 (2021-07-20)
Visceral leishmaniasis (VL) is a zoonotic protozoal vector-borne disease that is a major public health challenge. In Argentina, canine (CVL) and human visceral leishmaniasis (HVL) have recently emerged. There is a lack of standardised diagnostic tests for CVL, which hinders
J A C Guedes et al.
Bone, 115, 68-82 (2017-12-01)
The discovery of osteocalcin, a protein synthetized by osteoblasts, as a hormone that has positive effects on insulin resistance, contributed to support the concept of bone as an endocrine organ. However, very little is known about the molecular pathways involved
Giorgia Montalbano et al.
Nanomaterials (Basel, Switzerland), 10(9) (2020-09-02)
Bone-tissue regeneration induced by biomimetic bioactive materials is the most promising approach alternative to the clinical ones used to treat bone loss caused by trauma or diseases such as osteoporosis. The goal is to design nanostructured bioactive constructs able to
商品
A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
实验方案
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性，应注意防潮。
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