跳转至内容
Merck
CN

M0643

Sigma-Aldrich

Eagle最低必需培养基

With Earle′s salts, L-glutamine, and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture

别名:

MEM

登录查看公司和协议定价


About This Item

UNSPSC代码:
12352207
NACRES:
NA.75

质量水平

表单

powder

技术

cell culture | mammalian: suitable

组分

HEPES: no
Earle’s salts (5% CO2): yes
NaHCO3: no
phenol red: yes
L-glutamine: yes
sodium pyruvate: no

运输

ambient

储存温度

2-8°C

正在寻找类似产品? 访问 产品对比指南

一般描述

它含有19种氨基酸。必需氨基酸和非必需氨基酸;L-ala、L-asn、L-asp、L-glu、L-gly、L-pro和L-ser。可能跟适合于在血清浓度降低的情况下生长贴壁细胞,或作为开发无血清培养基的基础。
最小必需培养基Eagle包含糖、无机盐、水溶性维生素和必需氨基酸。它广泛用于许多细胞培养基研究的培养基配方。它是Eagle基础培养基(BME)的改良版本,大部分氨基酸的浓度是BME的两倍。

应用

最小必需培养基Eagle已用于培养成骨样细胞系MG-63、分散心室细胞和子宫内膜腺癌Ishikawa细胞。

数量

按照每升培养基9.6克粉末配制。

重悬

补充2.2 g/L碳酸氢钠。

储存分类代码

11 - Combustible Solids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

从最新的版本中选择一种:

分析证书(COA)

Lot/Batch Number

没有发现合适的版本?

如果您需要特殊版本,可通过批号或批次号查找具体证书。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

Tatsuma Yao et al.
Reproductive medicine and biology, 16(2), 99-117 (2017-12-21)
Cell culture technology has spread prolifically within a century, a variety of culture media has been designed. This review goes through the history, characteristics and current issues of animal-cell culture media. A literature search was performed on PubMed and Google
Is it time to reinvent basic cell culture medium?
Turney J McKee et al.
American journal of physiology. Cell physiology, 312(5), C624-C626 (2017-02-24)
Atomic layer deposited TiO2 films on an equiatomic NiTi shape memory alloy for biomedical applications
Abbas A, et al.
Journal of alloys and compounds, 161282-161282 (2021)
János Magyar et al.
European journal of pharmacology, 487(1-3), 29-36 (2004-03-23)
Concentration-dependent (10-1000 microM) effects of terpenoid phenol derivatives were studied on L-type Ca(2+) current in isolated canine and human ventricular cardiomyocytes using the whole-cell configuration of patch clamp technique. Carvacrol, thymol and eugenol suppressed peak Ca(2+) current at +5 mV
Sarah M Davis et al.
Molecular therapy. Nucleic acids, 21, 266-277 (2020-07-02)
Small interfering RNAs (siRNAs) have the potential to treat a broad range of diseases. siRNAs need to be extensively chemically modified to improve their bioavailability, safety, and stability in vivo. However, chemical modifications variably impact target silencing for different siRNA sequences

商品

Ensure optimal cell culture outcomes with ultrapure, endotoxin-free water. Learn about the impact of water impurities on mammalian cell cultures and how to achieve optimal cell growth.

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

实验方案

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

粉末培养基及盐混合物极具吸湿性,应注意防潮。

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门