产品名称
培养基 199, With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
form
powder
technique(s)
cell culture | mammalian: suitable
components
HEPES: no
Hanks’ salts (2% CO2): yes
sodium pyruvate: no
NaHCO3: no
L-glutamine: yes
phenol red: yes
shipped in
ambient
storage temp.
2-8°C
Quality Level
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Application
培养基199已用于：
- 测试和确定传感器在复杂环境中的pH灵敏度
- 作为卵母细胞采集的培养基
- 作为杜氏利什曼虫（LdBOB）原体培养基的组分，用于培养杜氏利什曼虫
- 内脏脂肪组织外植体
General description
培养基199是维生素、氨基酸和其他因素的组合，最初开发为用于原代外植体培养的完全确定的培养基配方。这种培养基添加适当的补充剂时具有广泛的适用性，特别是对于非转化细胞。培养基 199 广泛用于疫苗生产、原代胰腺外植体和晶状体组织的体外培养。
Preparation Note
按照每升培养基10.6克粉末配制。
补充0.35 g/L碳酸氢钠。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
动植物来源培养基
此项目有
Song Wang et al.
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Mohammadhossein Dabaghi et al.
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In many biological systems, pH can be used as a parameter to understand and study cell dynamics. However, measuring pH in live cell culture is limited by the sensor ion specificity, proximity to the cell surface, and scalability. Commercially available
Johannes Dittmann et al.
Journal of the mechanical behavior of biomedical materials, 77, 764-775 (2017-08-12)
The present study focused on the material characterisation of porcine oocytes by experiments combined with an inverse finite-element method (iFEM) approach. In doing so, two different deformation states, compression and indentation, were realised to enable the validation of the numerical
Alberto Stracuzzi et al.
Biomechanics and modeling in mechanobiology, 20(2), 751-765 (2021-02-04)
Probing mechanical properties of cells has been identified as a means to infer information on their current state, e.g. with respect to diseases or differentiation. Oocytes have gained particular interest, since mechanical parameters are considered potential indicators of the success
商品
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.
实验方案
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性，应注意防潮。
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