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生物来源
plant
质量水平
形式
saline suspension
标记范围
2-7 mg per mL
技术
affinity chromatography: suitable
基质
cross-linked 4% beaded agarose
基质活化
cyanogen bromide
基质附着
carboxyl
基质隔离区
8 atoms
适用性
suitable for chromatography
储存温度
2-8°C
应用
Methotrexate-agarose is used in protein chromatography, affinity chromatography, metabolic pathways and specialty resins. Methotrexate-agarose has been used to investigate the toxicity mechanism of mortality in adult buffalo flies caused by ingestion of folate analogues. Methotrexate-agarose has also been used to study the purification, cloning, and functional expression of dihydroneopterin triphosphate 2′-epimerase from Escherichia coli.
外形
Suspension in 1.0 M NaCl containing preservative
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
C Ahn et al.
The Journal of biological chemistry, 272(24), 15323-15328 (1997-06-13)
Dihydroneopterin triphosphate (H2NTP) 2'-epimerase from Escherichia coli catalyzes the epimerization of H2NTP to dihydromonapterin triphosphate (H2MTP). The enzyme was purified 954-fold to apparent homogeneity by a combination of ammonium sulfate fractionation and column chromatography of Cibacron blue 3GA dye ligand
T Endo et al.
The EMBO journal, 7(4), 1153-1158 (1988-04-01)
We have purified milligram amounts of an importable mitochondrial precursor protein [the presequence of yeast cytochrome oxidase subunit IV fused to mouse dihydrofolate reductase (DHFR)]. This has made it possible, for the first time, to perform detailed studies on the
Kazumi Urakawa et al.
European journal of pharmacology, 435(2-3), 237-244 (2002-02-01)
N-[[4-[(2,4-diaminopteridin-6-yl)methyl]-3,4-dihydro-2H-1,4-benzothiazin-7-yl]-carbonyl]-L-homoglutamic acid (MX-68), a derivative of methotrexate, was chemically designed to resist polyglutamation and to have a high affinity for dihydrofolate reductase, in an attempt to reduce the side effects of methotrexate. We confirmed that MX-68 did not undergo polyglutamation
Gottfried Wilharm et al.
BMC microbiology, 4, 27-27 (2004-07-14)
Pathogenic Yersinia species (Y. enterocolitica, Y. pestis, Y. pseudotuberculosis) share a type three secretion system (TTSS) which allows translocation of effector proteins (called Yops) into host cells. It is believed that proteins are delivered through a hollow needle with an
Ying Xu et al.
Journal of bacteriology, 185(18), 5519-5526 (2003-09-02)
Adapting metabolic enzymes of microorganisms to low temperature environments may require a difficult compromise between velocity and affinity. We have investigated catalytic efficiency in a key metabolic enzyme (dihydrofolate reductase) of Moritella profunda sp. nov., a strictly psychrophilic bacterium with
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Biofiles reviews innovative technologies for cancer research, reflecting the complexity of the disease.
Cancer research innovations address the complexity of the disease, providing advanced technologies for varied applications.
本期生物档案回顾了我们的一些最新和最具创新性的技术及其在癌症研究中的具体应用。在准备本期生物档案时，有人指出癌症极为复杂，要确定一个与其他主题完全无关的主题极为困难。。
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