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安全信息

M0268

Sigma-Aldrich

Eagle最低必需培养基

With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

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别名:
MEM
UNSPSC代码:
12352207
NACRES:
NA.75

质量水平

500

形式

powder

技术

cell culture | mammalian: suitable

组分

HEPES: no
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: no
L-glutamine: yes
sodium pyruvate: no

运输

ambient

储存温度

2-8°C

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相关类别

一般描述

Eagle最低必需培养基(MEM培养基)是使用最广泛的一种合成细胞培养基。MEM培养基已用于多种细胞的单层培养。通常用于在胎牛血清(FBS)、小牛或马血清存在下培养贴壁细胞，如成纤维细胞。

应用

Eagle最低必需培养基(MEM培养基)已用于：
  • 原代肝细胞的分离和培养
  • 培养人Ishikawa子宮內膜癌细胞(ATTC)和L-Wnt-3a细胞(ATCC)，用于分泌Wnt家族成员3a(Wnt-3a)配体
  • 培养AN3CA细胞（子宫内膜癌细胞系）和HEC1-B人细胞（子宫内膜腺癌细胞系）

特点和优势

  • Eagle最低必需培养基(MEM培养基)含有较高浓度的氨基酸，类似于哺乳动物培养细胞的蛋白组分。
  • 去除钙成分后，培养基可用于悬浮细胞培养。

数量

按照每升培养基9.5克粉末配制。

重悬

补充2.2 g/L碳酸氢钠。

其他说明

除了以粉末形式提供外，基本上与原始配方相同。

补充剂

产品编号
说明
价格

通常也和此产品一起购买

产品编号
说明
价格

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Does Product M0268, Minimum Essential Medium Eagle, contain phenol red?

    Yes, this product contains phenol red.  Phenol red is included in most medium as a visual pH indicator.  The medium should be changed when the color turns yellow (acidic) or purple (basic).

  4. What is the formulation of Product M0268, Minimum Essential Medium Eagle?

    The formulation for this product can be found on the product datasheet.

  5. Does Product M0268, Minimum Essential Medium Eagle, have an expiration date?

    Yes, this product has an expiration date of 36 months from the date of manufacture. The expiration date can be found on the Certificate of Analysis.

  6. Does Product M0268, Minimum Essential Medium Eagle, contain sodium bicarbonate?

    No, this product does not contain sodium bicarbonate. It is recommended to add 2.2 g sodium bicarbonate per Liter of medium. Use either Product No. S5761 (powder) or S8761 (7.5% solution).

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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商品

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

实验方案

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

粉末培养基制备说明

粉末培养基及盐混合物极具吸湿性，应注意防潮。

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