生物来源
Escherichia coli (O55:B5)
质量水平
形式
lyophilized powder
纯化方式
trichloroacetic acid extraction
杂质
1-10% Protein (Lowry)
颜色
white to yellow
溶解性
water: soluble
运输
ambient
储存温度
2-8°C
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一般描述
该产品是从大肠杆菌血清型O55：B5中通过TCA提取获得。源菌株为CDC 1644-70。LPS O55：B5已用于以1ng/ml的浓度刺激人腹膜巨噬细胞，以及以1-100ng/ml的浓度刺激马腹膜巨噬细胞。
应用
脂多糖（LPS）是革兰氏阴性细菌细胞壁的特征组分。脂多糖及其脂质 A 部分通过 Toll 样受体 4（TLR4）刺激先天免疫系统的细胞，Toll 样受体 4是 Toll 样受体蛋白家族的成员，其识别常见的病原体相关分子模式（PAMP）。
生化/生理作用
脂多糖（LPS）位于膜的外层，并且在非包封的菌株中暴露在细胞表面上。它们有助于外膜的完整性，并保护细胞免受胆汁盐和亲脂性抗生素的作用。
制备说明
该产品可溶于水（5mg/mL）或细胞培养基（1mg/mL），并产生浑浊的淡黄色溶液。在涡旋并升温至70-80 oC后，在盐水中可获得更加浓缩但仍然浑浊的溶液（20mg/ml）。脂多糖是一种能在各种溶剂中形成胶束的分子。在水和磷酸盐缓冲盐水中可观察到浑浊的溶液。通过有机溶剂无法获得更澄清的溶液。在甲醇会产生含有漂浮物的混浊悬浮液，而在水中则产生均匀的浑浊溶液。
相关产品
产品编号
说明
价格
警示用语：
Danger
危险声明
危险分类
Acute Tox. 2 Oral
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
O Schulz et al.
Mucosal immunology, 7(4), 892-904 (2013-12-19)
Lymphoid organ hypertrophy is a hallmark of localized infection. During the inflammatory response, massive changes in lymphocyte recirculation and turnover boost lymphoid organ cellularity. Intriguingly, the exact nature of these changes remains undefined to date. Here, we report that hypertrophy
Yu-Yuan Chen et al.
PloS one, 9(12), e115232-e115232 (2014-12-18)
Invertebrates rely on an innate immune system to combat invading pathogens. The system is initiated in the presence of cell wall components from microbes like lipopolysaccharide (LPS), β-1,3-glucan (βG) and peptidoglycan (PG), altogether known as pathogen-associated molecular patterns (PAMPs), via
Xiao-Wen Lin et al.
PloS one, 8(6), e67633-e67633 (2013-06-27)
Toll-like receptors (TLRs) play a pivotal role in the defense against invading pathogens by detecting pathogen-associated molecular patterns (PAMPs). TLR4 recognizes lipopolysaccharides (LPS) in the cell walls of Gram-negative bacteria, resulting in the induction and secretion of proinflammatory cytokines such
Oscar Pizano-Martínez et al.
World journal of gastroenterology, 17(33), 3830-3835 (2011-10-12)
To demonstrate that CD14⁺ cells are an important source of the growth factor YKL-40 in acute and chronic liver damage. Rats were inoculated with one dose of CCl(4) to induce acute damage. Liver biopsies were obtained at 0, 6, 12
J T Frieling et al.
Antimicrobial agents and chemotherapy, 41(7), 1439-1443 (1997-07-01)
The in vitro production of interleukin-1beta (IL-1beta), IL-6, and the IL-1 receptor antagonist (IL-1ra) in whole blood upon stimulation with different bacterial strains was measured to study the possible relationship between disease severity and the cytokine-inducing capacities of these strains.
商品
Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.
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