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文件

L3540

Lowry试剂

Name: Lowry试剂
Brand: SIGMA

别名:

Copper(II) tartrate Mix

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About This Item

UNSPSC代码:

12352200

NACRES:

NA.32

质量水平

100

一般描述

由于灵敏、简单且精确，几十年来Lowry法一直是定量测定可溶性蛋白的首选方案。微量Lowry 法的Peterson′改进法是一种简单、快速、客观、适用性更广的方法。在这种操作方法中，先是将十二烷基硫酸钠（SDS）与Lowry 试剂混合，从而使较为不溶的脂蛋白溶解。

应用

Lowry试剂已被用于定量测定胞外聚合物物质（EPS）中的蛋白质含量。它还用于定量测定黑腹果蝇的总蛋白和线粒体蛋白。

用于蛋白质测定。可利用SDS促进相对不溶的脂蛋白的溶解。

其他说明

含有Lowry试剂和十二烷基硫酸钠的干粉混合物。

重悬

用去离子水重新配制成40mL。

TP0300

总蛋白试剂盒，Micro Lowry，Peterson改良版

象形图

GHS02,GHS05,GHS07

警示用语：

Danger

危险声明

H228,H302,H314,H412

预防措施声明

P210 - P260 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338

危险分类

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Flam. Sol. 2 - Skin Corr. 1B

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

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Environmental and genetic perturbations reveal different networks of metabolic regulation

Greenberg A, et al.

Molecular Systems Biology, 7(1), 563-563 (2011)

A simplification of the protein assay method of Lowry et al. which is more generally applicable

Peterson GL

Analytical Biochemistry, 83(2), 346-356 (1977)

Ginger extract inhibits biofilm formation by Pseudomonas aeruginosa PA14

Kim HS and Park HD

PLoS ONE, 8(9), e76106-e76106 (2013)

Raffinose, a plant galactoside, inhibits Pseudomonas aeruginosa biofilm formation via binding to LecA and decreasing cellular cyclic diguanylate levels

Kim HS, et al.

Scientific reports, 6(1), 25318-25318 (2016)

Synergistic control of membrane biofouling using linoleic acid and sodium hypochlorite.

So-Young Ham et al.

Chemosphere, 268, 128802-128802 (2020-11-10)

Biofouling is a major operational problem in the reverse osmosis (RO) process, affecting the membrane performance. Although sodium hypochlorite (NaOCl) is used to chemically clean the biofouled membranes, high concentrations of NaOCl cause morphological and chemical damage to the RO

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实验方案

Protein Determination Modified Lowry Method

To standardize a procedure for the determination of protein by modified Lowry.

Protein Determination by the Warburg-Christian Method

To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.

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