生物来源
rabbit muscle
质量水平
类型
Type XI
形式
lyophilized powder
比活
600-1,200 units/mg protein
分子量
140 kDa
组成
protein, 90-100%
储存条件
(Keep container tightly closed in a dry and well-ventilated place)
技术
activity assay: suitable
颜色
white
异质活性
pyruvate kinase, myokinase, malic dehydrogenase, glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase and α-glycerophosphate dehydrogenase ≤0.01%
储存温度
−20°C
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一般描述
研究领域： 细胞信号传导
乳酸脱氢酶（LDH）的总分子量为140kDa，由4个亚基组成，称为M亚基（肌肉）和H亚基（心脏）。这些亚基可以以5种组合（M4、M3H1、M2H2、MH3和H4）中的任何一种进行混合。 骨骼肌含有LDH，其主要是M4，含有少量M3H和微量的H2H2。 H和M亚基的分子量非常相似，但氨基酸组成十分不同。兔肌肉LDH在pH5.0的乙酸盐-氯化物中可解离为二聚体物质（MW = ~70kDa），该解离是可逆的。Biochemistry, 13, 3527-3531 (1974). 氧化乙醛酸和乳酸。
等电点：8.4-8.6
最适pH值：7.5.
等电点：8.4-8.6
最适pH值：7.5.
应用
兔肌肉L-乳酸脱氢酶已用于：
- 作为肌纤维ATP酶活性和等长稳态张力测量用活化和松弛液的组分
- 测定刚果锥虫(Trypanosoma congolense)丙酮酸激酶活性
- 测定水稻质体丙酮酸酶OsPK2的丙酮酸激酶(PK)活性
生化/生理作用
肌型乳酸脱氢酶(LDH)参与多种代谢途径，是无氧糖酵解的必需活性成分。乳酸脱氢酶活性可被抗坏血酸抑制。乳酸脱氢酶可从NADH中再生烟酰胺腺嘌呤二核苷酸(NAD+)，工业上可用于生产聚乳酸。在无氧条件下，LDH 参与发酵反应，催化丙酮酸生成乳酸，并将烟酰胺腺嘌呤二核苷酸 (NADH) 氧化为NAD+。因此，LDH 可介导NAD+基础无氧糖酵解途径。通过这一途径，LDH 有助于在缺氧的情况下维持细胞的生理和生化功能。
其还可催化其他L-2-羟基单羧酸的氧化。
单位定义
在pH 7.5、37 °C条件下，一单位酶每分钟可将1.0 μmole丙酮酸还原成L-乳酸。
分析说明
通过缩二脲法测定的蛋白质。
抑制剂
抗体
酶
产品编号
说明
价格
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
WGK
WGK 1
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
Belén Torrado et al.
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We describe a method based on a pair of transmission filters placed in the emission path of a microscope to resolve the emission wavelength of every point in an image. The method can be applied to any type of imaging
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Biochemistry (2022)
Matthew Warren Eggert et al.
Applied biochemistry and biotechnology, 165(2), 676-686 (2011-06-01)
In order to evaluate the effectiveness of L: -lactate dehydrogenase (LDH) from rabbit muscle as a regenerative catalyst of the biologically important cofactor nicotinamide adenine dinucleotide (NAD), the kinetics over broad concentrations were studied to develop a suitable kinetic rate
John Jeshurun Michael et al.
Journal of the American Heart Association, 5(3), e002777-e002777 (2016-03-24)
We hypothesized that the functional effects of R206L-a rat analog of the dilated cardiomyopathy (DCM) mutation R205L in human cardiac troponin T (TnT)-were differently modulated by myosin heavy chain (MHC) isoforms and T204E, a protein kinase C (PKC) phosphomimic of
Percy J Russell et al.
Journal of enzyme inhibition and medicinal chemistry, 19(1), 91-98 (2004-06-19)
Muscle-type LDH (LDH-m4) activity is critical for efficient anaerobic glycolysis. The results here show that rabbit LDH-M4 is inhibited by concentrations of ascorbate normally found in tissues. Aldolase and muscle G-actin were found to protect and to reverse inhibitions of
商品
For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein
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