biological source
rabbit muscle
Quality Level
type
Type XI
form
lyophilized powder
specific activity
600-1,200 units/mg protein
mol wt
140 kDa
composition
protein, 90-100%
storage condition
(Keep container tightly closed in a dry and well-ventilated place)
technique(s)
activity assay: suitable
color
white
foreign activity
pyruvate kinase, myokinase, malic dehydrogenase, glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase and α-glycerophosphate dehydrogenase ≤0.01%
storage temp.
−20°C
General description
研究领域: 细胞信号传导
乳酸脱氢酶(LDH)的总分子量为140kDa,由4个亚基组成,称为M亚基(肌肉)和H亚基(心脏)。这些亚基可以以5种组合(M4、M3H1、M2H2、MH3和H4)中的任何一种进行混合。 骨骼肌含有LDH,其主要是M4,含有少量M3H和微量的H2H2。 H和M亚基的分子量非常相似,但氨基酸组成十分不同。兔肌肉LDH在pH5.0的乙酸盐-氯化物中可解离为二聚体物质(MW = ~70kDa),该解离是可逆的。Biochemistry, 13, 3527-3531 (1974). 氧化乙醛酸和乳酸。
等电点:8.4-8.6
最适pH值:7.5.
等电点:8.4-8.6
最适pH值:7.5.
Application
兔肌肉L-乳酸脱氢酶已用于:
- 作为肌纤维ATP酶活性和等长稳态张力测量用活化和松弛液的组分
- 测定刚果锥虫(Trypanosoma congolense)丙酮酸激酶活性
- 测定水稻质体丙酮酸酶OsPK2的丙酮酸激酶(PK)活性
Biochem/physiol Actions
其还可催化其他L-2-羟基单羧酸的氧化。
肌型乳酸脱氢酶(LDH)参与多种代谢途径,是无氧糖酵解的必需活性成分。乳酸脱氢酶活性可被抗坏血酸抑制。乳酸脱氢酶可从NADH中再生烟酰胺腺嘌呤二核苷酸(NAD+),工业上可用于生产聚乳酸。在无氧条件下,LDH 参与发酵反应,催化丙酮酸生成乳酸,并将烟酰胺腺嘌呤二核苷酸 (NADH) 氧化为NAD+。因此,LDH 可介导NAD+基础无氧糖酵解途径。通过这一途径,LDH 有助于在缺氧的情况下维持细胞的生理和生化功能。
Analysis Note
通过缩二脲法测定的蛋白质。
Other Notes
在pH 7.5、37 °C条件下,一单位酶每分钟可将1.0 μmole丙酮酸还原成L-乳酸。
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
商品
For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein
相关内容
QC Methods
Matthew Warren Eggert et al.
Applied biochemistry and biotechnology, 165(2), 676-686 (2011-06-01)
In order to evaluate the effectiveness of L: -lactate dehydrogenase (LDH) from rabbit muscle as a regenerative catalyst of the biologically important cofactor nicotinamide adenine dinucleotide (NAD), the kinetics over broad concentrations were studied to develop a suitable kinetic rate
Farhana A and Lappin. SL
Biochemistry (2022)
Belén Torrado et al.
Biomedical optics express, 12(7), 3760-3774 (2021-08-31)
We describe a method based on a pair of transmission filters placed in the emission path of a microscope to resolve the emission wavelength of every point in an image. The method can be applied to any type of imaging
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| L1254-50KU | 04061832730547 |
| L1254-5KU | 04061832730554 |
| L1254-25KU | 04061833949269 |
| L1254-1KU | 04061833949252 |
| L1254-100KU | 04061835545902 |
