推荐产品
生物来源
rabbit muscle
质量水平
类型
Type XI
表单
lyophilized powder
比活
600-1,200 units/mg protein
分子量
140 kDa
组成
protein, 90-100%
储存条件
(Keep container tightly closed in a dry and well-ventilated place)
技术
activity assay: suitable
颜色
white
异质活性
pyruvate kinase, myokinase, malic dehydrogenase, glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase and α-glycerophosphate dehydrogenase ≤0.01%
储存温度
−20°C
正在寻找类似产品? 访问 产品对比指南
一般描述
研究领域: 细胞信号传导
乳酸脱氢酶(LDH)的总分子量为140kDa,由4个亚基组成,称为M亚基(肌肉)和H亚基(心脏)。这些亚基可以以5种组合(M4、M3H1、M2H2、MH3和H4)中的任何一种进行混合。 骨骼肌含有LDH,其主要是M4,含有少量M3H和微量的H2H2。 H和M亚基的分子量非常相似,但氨基酸组成十分不同。兔肌肉LDH在pH5.0的乙酸盐-氯化物中可解离为二聚体物质(MW = ~70kDa),该解离是可逆的。Biochemistry, 13, 3527-3531 (1974). 氧化乙醛酸和乳酸。
等电点:8.4-8.6
最适pH值:7.5.
等电点:8.4-8.6
最适pH值:7.5.
应用
兔肌肉L-乳酸脱氢酶已用于:
- 作为肌纤维ATP酶活性和等长稳态张力测量用活化和松弛液的组分
- 测定刚果锥虫(Trypanosoma congolense)丙酮酸激酶活性
- 测定水稻质体丙酮酸酶OsPK2的丙酮酸激酶(PK)活性
生化/生理作用
其还可催化其他L-2-羟基单羧酸的氧化。
肌型乳酸脱氢酶(LDH)参与多种代谢途径,是无氧糖酵解的必需活性成分。乳酸脱氢酶活性可被抗坏血酸抑制。乳酸脱氢酶可从NADH中再生烟酰胺腺嘌呤二核苷酸(NAD+),工业上可用于生产聚乳酸。在无氧条件下,LDH 参与发酵反应,催化丙酮酸生成乳酸,并将烟酰胺腺嘌呤二核苷酸 (NADH) 氧化为NAD+。因此,LDH 可介导NAD+基础无氧糖酵解途径。通过这一途径,LDH 有助于在缺氧的情况下维持细胞的生理和生化功能。
单位定义
在pH 7.5、37 °C条件下,一单位酶每分钟可将1.0 μmole丙酮酸还原成L-乳酸。
分析说明
通过缩二脲法测定的蛋白质。
抑制剂
抗体
酶
产品编号
说明
价格
警示用语:
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 1
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Matthew Warren Eggert et al.
Applied biochemistry and biotechnology, 165(2), 676-686 (2011-06-01)
In order to evaluate the effectiveness of L: -lactate dehydrogenase (LDH) from rabbit muscle as a regenerative catalyst of the biologically important cofactor nicotinamide adenine dinucleotide (NAD), the kinetics over broad concentrations were studied to develop a suitable kinetic rate
Farhana A and Lappin. SL
Biochemistry (2022)
Belén Torrado et al.
Biomedical optics express, 12(7), 3760-3774 (2021-08-31)
We describe a method based on a pair of transmission filters placed in the emission path of a microscope to resolve the emission wavelength of every point in an image. The method can be applied to any type of imaging
John Jeshurun Michael et al.
Journal of the American Heart Association, 5(3), e002777-e002777 (2016-03-24)
We hypothesized that the functional effects of R206L-a rat analog of the dilated cardiomyopathy (DCM) mutation R205L in human cardiac troponin T (TnT)-were differently modulated by myosin heavy chain (MHC) isoforms and T204E, a protein kinase C (PKC) phosphomimic of
Jialin Fu et al.
JCI insight, 7(5) (2022-02-09)
Diabetic nephropathy (DN) arises from systemic and local changes in glucose metabolism and hemodynamics. We have reported that many glycolytic and mitochondrial enzymes, such as pyruvate kinase M2 (PKM2), were elevated in renal glomeruli of DN-protected patients with type 1
商品
For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门