一般描述
Krebs-Henseleit改良缓冲液是一种平衡盐溶液(BSS),用于细胞培养,可以为细胞提供正常细胞代谢所需的水和无机盐。此外,它提供了一个缓冲系统,以维持介质的最佳pH值,并作为灌溉、运输和稀释剂,以维持细胞内和细胞外的渗透平衡。BSS提供葡萄糖作为细胞代谢的能量来源。
应用
Krebs-Henseleit改良缓冲液已用于:
- 采集大鼠脑组织进行离体脑切片实验
- 测定大鼠肺动脉内皮细胞(PAEC)内钙和钠的含量
- 采集马的子宫和前肢血管样本
数量
配制成每升培养基中含粉末 9.6 克。
重悬
补充 2.1 g/L 碳酸氢钠。
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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实验方案
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性,应注意防潮。
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