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一般描述
与人IgG结合；不与其他人Igs结合。该抗体对人IgG重链上的决定簇具有特异性。在3％ PEG 6000存在时，该抗体可在液相中形成抗原-抗体复合物。
Monoclonal Anti-Human IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Immunoglobulins have two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is usually found as a monomer. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4. About 70 percent of the total immunoglobulin consists of IgG.
应用
利用碱性磷酸酶偶联的小鼠抗-人IgG单克隆抗体通过Elisa对健康个体血清中针对各种金黄色葡萄球菌抗原的抗体进行检测。将抗体在PBSt中按1:30000稀释，并在37度下与样品孵育2小时。Reactin是使用对硝基苯基磷酸酯底物（Sigma）进行开发的。
Monoclonal Anti-Human IgG (γ-chain specific) antibody produced in mouse has been used in enzyme linked immunosorbent assay (ELISA).
生化/生理作用
Immunoglobulin G (IgG) mainly helps in immune defense. IgG participates in hypersensitivity type II and type III.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Alain Townsend et al.
Nature communications, 12(1), 1951-1951 (2021-03-31)
Serological detection of antibodies to SARS-CoV-2 is essential for establishing rates of seroconversion in populations, and for seeking evidence for a level of antibody that may be protective against COVID-19 disease. Several high-performance commercial tests have been described, but these
Utility of the P 19 suppressor of gene-silencing protein for production of therapeutic antibodies in N icotiana expression hosts
Garabagi F, et al.
Plant Biotechnology Journal, 10(9), 1118-1128 (2012)
Charles Pilette et al.
Immunology and cell biology, 88(4), 486-493 (2010-01-20)
As previously reported by others for immunoglobulin (Ig)G, we observed that IgA can induce interleukin (IL)-10 expression in human monocytes. In this study, we explored the molecular mechanisms of IL-10 induction by IgA in monocytes and monocyte-derived dendritic cells (MD-DCs).
Maha Zohra Ladjemi et al.
The European respiratory journal, 45(4), 980-993 (2014-12-30)
Despite their relevance to mucosal defense, production of IgA and the function of lung B-cells remain unknown in chronic obstructive pulmonary disease (COPD). We assessed IgA synthesis in the lungs of COPD (n=28) and control (n=21) patients, and regulation of
Freydoun Garabagi et al.
Plant biotechnology journal, 10(9), 1118-1128 (2012-09-19)
To study how the P19 suppressor of gene-silencing protein can be used effectively for the production of therapeutic glycoproteins, the following factors were examined: the genetic elements used for expressing recombinant proteins; the effect of different P19 concentrations; compatibility of
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