一般描述
单克隆抗-人IgG2（小鼠 IgG1亚型）来源于小鼠骨髓瘤细胞与免疫小鼠脾细胞融合产生的杂交瘤。人IgG由四个亚类组成。
特异性
该抗体对人 IgG2 具有特异性，与其他 IgG 亚类不反应。该克隆已被 IUIS/WHO 研究挑选出为应用最广泛的 IgG2 特异性单克隆抗体。
应用
抗人IgG2单克隆抗体可用于酶联免疫吸附试验（ELISA）和蛋白质印迹。
也可用于
也可用于
- 印迹免疫固定法
- 免疫荧光法
- 血凝法
- 血凝抑制法
- 颗粒计数免疫分析法
- 细胞质IgG检测
生化/生理作用
抗多糖反应的主要是IgG2亚类，而蛋白质抗原产生的则是IgG1和IgG3抗体。脂多糖刺激外周血白细胞（PBL′s）中的IgG2反应。
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法规信息
常规特殊物品
Identification of immune biomarkers related to disease progression and treatment efficacy in human visceral leishmaniasis
Portela A S B, et al.
Immunobiology, 223(3), 303-309 (2018)
A conserved Leishmania hypothetical protein evaluated for the serodiagnosis of canine and human visceral and tegumentary leishmaniasis, as well as a serological marker for the posttreatment patient follow-up
Ribeiro P A, et al.
Diagnostic Microbiology and Infectious Disease, 92(3), 196-203 (2018)
Negative immunodiffusion test results obtained with sera of paracoccidioidomycosis patients may be related to low-avidity immunoglobulin G2 antibodies directed against carbohydrate epitopes
Neves AR, et al.
Clinical and Diagnostic Laboratory Immunology, 10(5), 802-807 (2003)
Z Lazarova et al.
The Journal of investigative dermatology, 114(1), 178-184 (2000-01-05)
Patients with one form of cicatricial pemphigoid have IgG antibasement membrane autoantibodies against laminin 5 (alpha3beta3gamma2). Although passive transfer of rabbit anti-laminin 5 IgG to neonatal mice has been shown to induce subepidermal blisters that mimic those in patients, it
D Thiagarajan et al.
ACR open rheumatology, 2(6), 344-356 (2020-05-12)
Immunoglobulin M antibodies against phosphorylcholine (anti-PCs) may be protective in atherosclerosis, cardiovascular disease (CVD), and systemic lupus erythematosus (SLE). We study immunoglobulin G1 (IgG1) and immunoglobulin G2 (IgG2) anti-PCs, with a focus on atherosclerosis and SLE. We determined anti-PCs by
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