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生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
IgG fraction of antiserum
抗体产品类型
secondary antibodies
克隆
polyclonal
表单
buffered aqueous solution
技术
indirect ELISA: 1:80,000
quantitative precipitin assay: 3.0-4.0 mg/mL
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
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一般描述
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Immunoglobulin have two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein, usually found as a monomer. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4. IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. About 70 % of the total immunoglobulin consists of IgG.
特异性
The antiserum is determined to be immunospecific for human IgG. Reactivity with light chains is observed.
应用
Anti-Human IgG (whole molecule) antibody produced in rabbit has been used in immunoprecipitation and enzyme linked immuno sorbent assay (ELISA).
Anti-Human IgG (whole molecule) antibody produced in rabbit was coated on Sepharose beads to immunoprecipitate IgG from HUVEC cultured conditioned medium.
生化/生理作用
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative
储存及稳定性
For continuous use, store at 2-8 °C. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is notrecommended.If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Adherence of platelets to in situ albumin-binding surfaces under flow conditions: role of surface-adsorbed albumin
Thakurta SG, et al.
Biomedical Materials (Bristol, England), 7(4), 045007-045007 (2012)
D Carvalho et al.
The Journal of clinical investigation, 97(1), 111-119 (1996-01-01)
IgG autoantibodies that bind human endothelial cells (AECA) were detected by ELISA in 30 of 42 samples of sera from patients with scleroderma. Pretreatment of human umbilical vein endothelial cells with AECA-positive scleroderma sera, or IgG purified from these sera
Anri Miyama et al.
International ophthalmology, 35(4), 575-586 (2014-09-06)
Immunoglobulin G (IgG) antibodies are involved in type II and type III hypersensitivity. We evaluated the relation between perennial allergic conjunctivitis and serum levels of specific IgG for cat allergens. A prospective study was conducted in patients with seasonal allergic
Wei Wang et al.
Journal of pharmaceutical sciences, 96(1), 1-26 (2006-09-26)
The number of therapeutic monoclonal antibody in development has increased tremendously over the last several years and this trend continues. At present there are more than 23 approved antibodies on the US market and an estimated 200 or more are
Linda Harkness et al.
Methods in molecular biology (Clifton, N.J.), 873, 33-51 (2012-04-25)
In 1998, a development occurred in stem cell biology with the first report of the derivation of a human embryonic stem cell (hESC) line. Since then a number of techniques have been used to derive and characterise hESCs. Here, we
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