一般描述
Histopaque®-1077是包含多蔗糖和泛影酸钠的溶液，密度调整到1.077 +/- 0.001 g/cm3。这一培养基帮助来自小体积全血的活的淋巴细胞快速恢复。这一技术适合于调节淋巴溶解和人类淋巴细胞抗原（HLA）2分型的细胞。它还可用作T-、B-和“空”淋巴细胞计数前的起始分离步骤。用来建立密度培养基，用于淋巴细胞和其他单核细胞的纯化。
应用
Histopaque®-1077 Hybri-Max™已用于分离：
- 外周血单个核细胞（PBMC）与肝素化血，梯度离心法
- 来自外周血的免疫细胞（淋巴细胞）
- PBMC与血液
法律信息
Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
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说明
价格
警示用语：
Danger
危险声明
危险分类
Resp. Sens. 1 - Skin Sens. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
法规信息
动植物来源培养基
Janet E Alexander et al.
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As in human populations, advances in nutrition and veterinary care have led to an increase in the lifespan of companion animals. Detrimental physiological changes occurring later in life must be understood before interventions can be made to slow or reduce
Carla Oseroff et al.
Proceedings of the National Academy of Sciences of the United States of America, 102(39), 13980-13985 (2005-09-21)
We have analyzed by ex vivo ELISPOT the anti-vaccinia cytotoxic T lymphocyte responses of peripheral blood mononuclear cells from humans vaccinated with Dryvax vaccine. More than 6,000 peptides from 258 putative vaccinia ORFs predicted to bind the common molecules of
Elliott D SoRelle et al.
eLife, 10 (2021-01-28)
Lymphoblastoid cell lines (LCLs) are generated by transforming primary B cells with Epstein-Barr virus (EBV) and are used extensively as model systems in viral oncology, immunology, and human genetics research. In this study, we characterized single-cell transcriptomic profiles of five
Mauricio S Caetano et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 25(24), 7576-7584 (2019-09-22)
Radiotherapy (RT) traditionally has been used for local tumor control in the treatment of cancer. The recent discovery that radiotherapy can have anticancer effects on the immune system has led to recognition of its ability to sensitize the tumor microenvironment
Joshua E Messinger et al.
mBio, 10(4) (2019-07-04)
Deciphering the molecular pathogenesis of virally induced cancers is challenging due, in part, to the heterogeneity of both viral gene expression and host gene expression. Epstein-Barr virus (EBV) is a ubiquitous herpesvirus prevalent in B-cell lymphomas of immune-suppressed individuals. EBV
实验方案
本疑难排查指南针对使用 Histopaque 时观察到的最常见错误来源提出解决方案，但这并不代表本指南是全面无遗的。
This troubleshooting guide addresses the most common sources of error observed when using Histopaque but is not meant to be a comprehensive list.
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