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Merck
CN

H8889

Histopaque®-1077 Hybri-Max

liquid, sterile-filtered, BioReagent, suitable for hybridoma

别名:

Histopaque 1077

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关于此项目

NACRES:
NA.75
UNSPSC Code:
12352201
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产品名称

Histopaque®-1077 Hybri-Max, liquid, sterile-filtered, BioReagent, suitable for hybridoma

sterility

sterile-filtered

product line

BioReagent

form

liquid

technique(s)

cell culture | hybridoma: suitable
single cell analysis: suitable

impurities

endotoxin, tested

density

1.077 at 25 °C (relative density)(lit.)

storage temp.

2-8°C

Quality Level

Application

Histopaque®-1077 Hybri-Max已用于分离:
  • 外周血单个核细胞(PBMC)与肝素化血,梯度离心法
  • 来自外周血的免疫细胞(淋巴细胞)
  • PBMC与血液

General description

Histopaque®-1077是包含多蔗糖和泛影酸钠的溶液,密度调整到1.077 +/- 0.001 g/cm3这一培养基帮助来自小体积全血的活的淋巴细胞快速恢复。这一技术适合于调节淋巴溶解和人类淋巴细胞抗原(HLA)2分型的细胞。它还可用作T-、B-和“空”淋巴细胞计数前的起始分离步骤。用来建立密度培养基,用于淋巴细胞和其他单核细胞的纯化。

Legal Information

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

存储类别

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

法规信息

动植物来源培养基
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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访问文档库

Joshua E Messinger et al.
mBio, 10(4) (2019-07-04)
Deciphering the molecular pathogenesis of virally induced cancers is challenging due, in part, to the heterogeneity of both viral gene expression and host gene expression. Epstein-Barr virus (EBV) is a ubiquitous herpesvirus prevalent in B-cell lymphomas of immune-suppressed individuals. EBV
Siddharth Sharma et al.
Cytometry. Part A : the journal of the International Society for Analytical Cytology, 73(2), 160-167 (2008-01-15)
Coulter volume is far more accurate measure of cell volume than forward angle light scatter. In this report, we have used Coulter volume to determine the mean cell volume and diameter of normal human peripheral blood cells and hematopoietic progenitor
Mauricio S Caetano et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 25(24), 7576-7584 (2019-09-22)
Radiotherapy (RT) traditionally has been used for local tumor control in the treatment of cancer. The recent discovery that radiotherapy can have anticancer effects on the immune system has led to recognition of its ability to sensitize the tumor microenvironment
Elliott D SoRelle et al.
eLife, 10 (2021-01-28)
Lymphoblastoid cell lines (LCLs) are generated by transforming primary B cells with Epstein-Barr virus (EBV) and are used extensively as model systems in viral oncology, immunology, and human genetics research. In this study, we characterized single-cell transcriptomic profiles of five
Janet E Alexander et al.
The journals of gerontology. Series A, Biological sciences and medical sciences, 73(6), 720-728 (2017-11-11)
As in human populations, advances in nutrition and veterinary care have led to an increase in the lifespan of companion animals. Detrimental physiological changes occurring later in life must be understood before interventions can be made to slow or reduce

实验方案

This troubleshooting guide addresses the most common sources of error observed when using Histopaque but is not meant to be a comprehensive list.

本疑难排查指南针对使用 Histopaque 时观察到的最常见错误来源提出解决方案,但这并不代表本指南是全面无遗的。

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