H6875
Hippuryl-L-phenylalanine
别名:
Hippuryl-Phe, N-Benzoyl-Gly-Phe
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关于此项目
经验公式（希尔记法）:
C18H18N2O4
化学文摘社编号:
分子量:
326.35
UNSPSC Code:
12352204
NACRES:
NA.32
PubChem Substance ID:
EC Number:
212-016-7
Beilstein/REAXYS Number:
2167818
MDL number:
InChI key
CCLJGZGVIQBNDH-HNNXBMFYSA-N
InChI
1S/C18H18N2O4/c21-16(12-19-17(22)14-9-5-2-6-10-14)20-15(18(23)24)11-13-7-3-1-4-8-13/h1-10,15H,11-12H2,(H,19,22)(H,20,21)(H,23,24)/t15-/m0/s1
SMILES string
OC(=O)[C@H](Cc1ccccc1)NC(=O)CNC(=O)c2ccccc2
assay
≥98% (TLC)
form
powder
solubility
acetic acid: 50 mg/mL, clear, colorless
storage temp.
−20°C
Quality Level
General description
Substrate for carboxypeptidase A
Application
Hippuryl-L-phenylalanine has been used as a substrate for screening carboxypeptidase activity in Trogoderma granarium, Bactrocera oleae Gmelin and Apodiphus amygdali.
Biochem/physiol Actions
Hippuryl-L-phenylalanine is a substrate for carboxypeptidase A enzyme.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Determination of kininase I and kininase II activities in human urine by high-performance liquid chromatography.
G Porcelli et al.
Journal of chromatography, 414(2), 423-428 (1987-03-06)
E Normant et al.
Neuropeptides, 30(1), 13-17 (1996-02-01)
We have designed two radioactive substrates, hippuryl-L-[3H]phenylalanine and 3-(p-hydroxy, m-[125I]phenyl)propionic acid ([125I]Bolton reagent) derivative of L-arginyl-L-phenylalanine, i.e. [125I]BRF, for a highly sensitive assay of carboxypeptidase A (CPA) activity. After cleavage of the C-terminal phenylalanine residue by CPA, the radioactive product
J H Cho et al.
Biochemistry, 40(34), 10197-10203 (2001-08-22)
We have investigated the function of Tyr248 using bovine wild-type CPA and its Y248F and Y248A mutants to find that the K(M) values were increased by 4.5-11-fold and the k(cat) values were reduced by 4.5-10.7-fold by the replacement of Tyr248
Product stabilization of the two forms of carboxypeptidase A from buffalo pancreas
Sud M and Dua RD
Journal of Biosciences, 9(1-2), 83-90 (1985)
Digestive proteolytic activity in Apodiphus amygdali Germar (Hemiptera: Pentatomidae): effect of endogenous inhibitors
Ramzi S and Zibaee A
Journal of Entomological and Acarological Research, 46(2), 35-41 (2014)
实验方案
Carboxypeptidase A activity measured via continuous spectrophotometric rate determination assay with hippuryl-L-phenylalanine substrate.
在测定羧肽酶A活性时，使用马尿酸-L-苯丙氨酸在254nm处进行连续分光光度法测定。羧肽酶A水解C末端残基上的肽键。
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