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应用
透明质酸酶是一种来自牛睾丸的酶。准备用于体外培养的卵子时,可利用这种酶去除小鼠胚胎分离物或新排卵中的卵丘物质。
促性腺激素用于使20只雌性小鼠超排卵。然后小鼠进行交配,获得并计数着床前期胚胎。然后胚胎使用透明质酸酶处理,知道其中所有积云物质都从胚胎里去除掉。胚胎被培育,每天查看,记录胚胎发育进展。
促性腺激素用于使20只雌性小鼠超排卵。然后小鼠进行交配,获得并计数着床前期胚胎。然后胚胎使用透明质酸酶处理,知道其中所有积云物质都从胚胎里去除掉。胚胎被培育,每天查看,记录胚胎发育进展。
生化/生理作用
这些酶可随机裂解透明质酸、软骨素和硫酸软骨素中的 β-N-乙酰己糖胺-[1→4] 糖苷键。
透明质酸酶可降解透明质酸,并已发现可在癌症进展期间会被不适当地调节。
单位定义
在 37 ºC、pH 5.35 条件下,一单位每分钟可降解 0.75 μg 多糖透明质酸(通过比浊吸光度测量(λ: 600nm),与 BSA 复合 45 分钟后测量)。
制备说明
用3 ml M2培养基(货号M 7167或M 5910)或去离子水复溶1瓶透明质酸酶,获得10 mg/ml的储备溶液。用M2培养基稀释储备溶液,至约300 μg/ml最终工作浓度。
警示用语:
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
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Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture
用于转基因小鼠胚胎培养的小鼠胚胎培养基及经过胚胎培养验证的试剂
实验方案
To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.
在测定透明质酸酶活性时,使用了一种在600 nm处的比浊测定法进行测定。在pH 5.35、37 °C下,一单位的透明质酸酶活性可每分钟引起0.330的吸光值改变。
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