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安全信息

H3631

Sigma-Aldrich

透明质酸酶 来源于牛睾丸

Type VI-S, lyophilized powder, 3,000-15,000 units/mg solid

别名:

玻璃糖醛酸酶, 玻璃酸酶

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About This Item

CAS号:
37326-33-3
EC 号:
3.2.1.35 (BRENDA, IUBMB)
EC 号:
253-464-3
MDL编号:
MFCD00131351
UNSPSC代码:
12352204
NACRES:
NA.54

生物来源

bovine testis

质量水平

200

偶联物

conjugate (Glucosaminoglycan)

类型

Type VI-S

形式

lyophilized powder

比活

3,000-15,000 units/mg solid

分子量

~55 kDa (four subunits of 14 kDa each)

UniProt登记号

Q5E985
Q8SQG8

相关疾病

cancer

储存温度

−20°C

基因信息

cow ... HYAL1(515397) , HYAL2(281838)

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相关类别

生化/生理作用

这些酶可随机裂解透明质酸、软骨素和硫酸软骨素中的 β-N-乙酰己糖胺-[1→4] 糖苷键。
透明质酸酶可降解透明质酸，并已发现可在癌症进展期间会被不适当地调节。

单位定义

在 37 ºC、pH 5.35 条件下，一单位每分钟可降解 0.75 μg 多糖透明质酸（通过比浊吸光度测量(λ: 600nm)，与 BSA 复合 45 分钟后测量）。

制备说明

色谱纯化产品

其他说明

更多关于复杂碳水化合物分析酶的信息，请见www.sigma-aldrich.com/enzymeexplorer

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H334

预防措施声明

P261 - P284 - P501

危险分类

Resp. Sens. 1

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

动植物源性产品

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Tongcui Ma et al.
eLife, 11 (2022-07-06)
High-parameter single-cell phenotyping has enabled in-depth classification and interrogation of immune cells, but to date has not allowed for glycan characterization. Here, we develop CyTOF-Lec as an approach to simultaneously characterize many protein and glycan features of human immune cells
Dong-Ru Ho et al.
International journal of urology : official journal of the Japanese Urological Association, 18(7), 525-531 (2011-05-25)
To investigate how hyaluronic acid (HA) affects nerve growth factor (NGF) production and bladder overactivity in a cyclophosphamide (CYP)-induced cystitis rat model. Female Sprague-Dawley rats received three intermittent intraperitoneal injections of CYP (75 mg/kg) or saline. Before or after CYP injection
Tongcui Ma et al.
eLife, 9 (2020-05-27)
The female reproductive tract (FRT) is the most common site of infection during HIV transmission to women, but viral remodeling complicates characterization of cells targeted for infection. Here, we report extensive phenotypic analyses of HIV-infected endometrial cells by CyTOF, and
Shared decision making, thrombotic thrombocytopenic purpura, and caplacizumab.
Senthil Sukumar et al.
American journal of hematology, 95(4), E76-E77 (2020-01-03)
Nathan V Welham et al.
Journal of speech, language, and hearing research : JSLHR, 52(4), 1008-1020 (2009-07-31)
To develop and evaluate a rat excised larynx model for the measurement of acoustic, aerodynamic, and vocal fold vibratory changes resulting from vocal fold scar. Twenty-four 4-month-old male Sprague-Dawley rats were assigned to 1 of 4 experimental groups: chronic vocal
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实验方案

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

透明质酸酶的酶法测定 (3.2.1.35)

在测定透明质酸酶活性时，使用了一种在600 nm处的比浊测定法进行测定。在pH 5.35、37 °C下，一单位的透明质酸酶活性可每分钟引起0.330的吸光值改变。

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