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一般描述
平衡盐溶液（BSS）用作冲洗、运输和稀释液体，同时维持细胞内和细胞外的渗透平衡。它还为细胞提供正常细胞代谢所必需的水和某些大块无机离子。BSS与碳水化合物（如葡萄糖）结合时，是细胞代谢的主要能量来源。此外，它还提供了一个缓冲系统，以维持培养基的生理pH值范围（7.2-7.6）。
应用
Hanks平衡盐已用于：
- 用于在胰腺血管分离过程中稀释消化组织
- 用于小鼠肠粘膜细胞裂解和超声前清洗
- 用作浸渍液，测试从墨鱼骨提取的多(ε-聚己内酯)-涂覆羟基磷灰石支架的体外生物活性
数量
配制成每升培养基中含粉末 9.7 克。
重悬
补充 0.35 g/L 碳酸氢钠。
其他说明
加入碳酸氢钠后调节 pH 值。使用干细胞或细胞克隆的研究人员通常更喜欢不含酚红的培养基。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
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实验方案
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性，应注意防潮。
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