产品名称
高尔基体分离试剂盒, sufficient for 50 g (tissue)
usage
sufficient for 50 g (tissue)
technique(s)
fractionation: suitable
shipped in
wet ice
storage temp.
2-8°C
Quality Level
Analysis Note
这一款高尔基体分离试剂盒采用大鼠肝脏样本进行了优化，并在大鼠肾脏、脾脏和心脏上进行过测试。
Application
高尔基体分离试剂盒可用于通过不连续密度梯度分离法从哺乳动物软组织中分离高尔基膜。
General description
高尔基体分离试剂盒提供了一种通过不连续密度梯度分离法从哺乳动物软组织中分离高尔基膜的方法。通过测定UDP-半乳糖转移酶的活性，或者采用适当的抗体免疫检测B-COP或GM130等高尔基体特异性标志物蛋白，来测定高尔基体的富集程度（货号：分别为G6160和G7295）。其他细胞器的分离可采用Sigma提供的相应标志物检测试剂盒检测（货号：CS0780、CYTOCOX1、CY0100和CAT100）。
存储类别
10 - Combustible liquids
wgk
WGK 3
法规信息
常规特殊物品
低风险生物材料
此项目有
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商品
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
亚细胞组分离心分离是一种常用技术，广泛适用于多种类型的细胞和组织。由于不同细胞器的大小、形状和密度不同，因此通过离心，可从温和均质的样品中轻松分离和纯化细胞器组分。
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