GE29-0486-84

HiTrap^® Desalting

Name: HiTrap<SUP>®</SUP> Desalting
Brand: SIGMA

Cytiva 29-0486-84, pack of 5 mL

别名:

Sephadex G-25 Superfine column, chromatography tube, cytiva 29048684, prepacked column

登录查看组织和合同定价。

选择尺寸

关于此项目

NACRES:

NA.56

UNSPSC Code:

23151816

主要文件

查看所有文档

技术服务

需要帮助？我们经验丰富的科学家团队随时乐意为您服务。

让我们为您提供帮助

技术服务

需要帮助？我们经验丰富的科学家团队随时乐意为您服务。

让我们为您提供帮助

产品名称

HiTrap^® Desalting, Cytiva 29-0486-84, pack of 5 mL

packaging

pack of 5 mL

manufacturer/tradename

Cytiva 29-0486-84

availability

not available in North America

storage condition

dry media

parameter

42 psi

bed size

16 mm × 25 mm

bed volume

5 mL

column I.D.

16 mm

matrix

cross-linked dextran

particle size

>20 μm (dry)

cleaning

2-13

working range

2-13

suitability

suitable for bioprocess medium

Application

Gel filtration has successfully been employed for size based separations of macromolecules since the late 1950s. Gel filtration, or size exclusion chromatography, may be performed in three principally different modes, depending on the size differences of the solutes to be separated (i) group separation (ii) fractionation (iii) determination of molecular mass (distributions) When the size difference is large, i.e. a factor of more than 10, we talk about group separation (e.g. desalting, buffer exchange) and when the size difference is small, i.e. a factor of 2 to 5, we talk about fractionation. The third mode is analytical gel filtration, e.g. determination of molecular mass distributions, where an array of molecular masses is to be separated. These different modes put different requirements on the equipment and media used and will yield widely different productivity in terms of information or material processed.

Features and Benefits

Handles sample volumes of 1.5 mL, or larger volumes by connecting up to five columns in series.
Replaces dialysis as the method of choice for desalting.
Desalting requires less than one minute due to the high rigidity of Sephadex G-25 and the optimized packing technology.

General description

HiTrap Desalting is a prepacked, ready to use column for group separation between high and low molecular weight substances, i.e., buffer exchange prior to or after different chromatographic steps, removal of low molecular weight contaminants or removal of reagents to terminate a reaction.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

Legal Information

HiTrap is a registered trademark of Cytiva

pictograms

GHS02

signalword

Warning

hcodes

H226

pcodes

P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501

存储类别

3 - Flammable liquids

法规信息

新产品

此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our 文件 section.

如需帮助，请联系客户支持

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

商品

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Automated Antibody Purification with ÄKTA Systems

Designing purification methods for antibodies ensures quality and quantity adequacy for research and industrial purposes.

Purification of GST-tagged Protein

GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.

查看所有结果