物料
bed support (polyamide)
glass column
glass fiber (polypropylene reinforced)
分子量
10000-600000 Da (globular proteins)
包装
pkg of 1 ea
制造商/商品名称
Cytiva 28-9893-35
参数
<1.0 mL/min flow rate
<5 mL sample volume
0.3 Mpa (42 psi)
柱床体积
120 mL
色谱柱长度 × 内径
60 cm × 16 mm
基质
agarose dextran composite
dextran-agarose particle platform
polymeric
基质活性基团
dextran-agarose phase
平均零件尺寸
34 μm
cleaning
1-14
working range
3-12
分离技术
size exclusion (SEC)
相关类别
一般描述
Superdex®制备级(pg)是通过葡聚糖与高度交联琼脂糖的共价结合产生的。这些介质的分离性质由葡聚糖组分决定。陡峭的选择性曲线为分子量范围内的多肽和蛋白质提供了优良的分辨能力：Mr < 10 000 (Superdex® 30 pg)、Mr 3 000至70 000 (Superdex® 75 pg)以及Mr 10 000 600 000 (Superdex® 200 pg)。该培养基结合了高机械强度和高亲水性，允许高流速和极低的非特异性相互作用。
特点和优势
- 分辨率高，运行时间短，回收率良好。
- 高流量下的高容量。
- 分离可以扩展至生产水平。
- 预先包装实现了方便和再现性。
- 1/16”配件方便连接到AKTA设计系统。
适用性
储存及稳定性
分析说明
法律信息
警示用语：
Warning
危险声明
储存分类代码
3 - Flammable liquids
商品
This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from Cytiva.
Understanding pressure drop in chromatography systems is crucial for optimal functionality across different parts of the system.
How to optimize purification of histidine-tagged proteins using Cytiva products.
实验方案
This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap® Protein G HP from Cytiva for the capture step.
This page shows the purification strategy of capture, intermediate purification, and polishing (CIPP) in affinity chromatography using Cytiva products.
Cytiva的Superdex是由葡聚糖和琼脂糖的复合基础基质组成的SEC介质。本页面介绍如何使用superdex执行分离任务。
Superdex from Cytiva are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.
相关内容
Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.
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