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GE28-9365-51

Hisprep™ Fast Flow 16/10

Cytiva 28-9365-51

别名:

16/10 Hisprep, Hisprep Column, Hisprep Fast Flow Column

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About This Item

UNSPSC代码:
23151817
NACRES:
NA.56

包装

pkg of 1 ea

制造商/商品名称

Cytiva 28-9365-51

参数

1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi

柱床尺寸

16 mm × 100 mm

柱床体积

20 mL

色谱柱内径

16 mm

基质

6% cross-linked agarose

粒径

45-165 μm

平均直径

90 μm

cleaning

2-14(Ni2+-stripped medium.)

working range

3-12(Ni2+-stripped medium.)

容量

~40 mg binding capacity(histidine-tagged protein)

适用性

suitable for bioprocess medium

相关类别

一般描述

HisPrep™ FF 16/10 is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow. The column is an excellent choice for preparative, scale-up purification of histidine-tagged recombinant proteins.

HisPrep™ FF 16/10 columns are prepacked with Ni Sepharose™ 6 Fast Flow for scale-up purification of histidine-tagged proteins. Ni Sepharose™ 6 Fast Flow consists of 90 ?m beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.

特点和优势

  • Optimized for convenient scale-up purification of histidine-tagged proteins.
  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
  • Negligible leakage of Ni2+.
  • High binding capacity, approx. 40 mg/mL medium.
  • Convenient and time-saving 20 mL prepacked HiPrep™ format.

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他说明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律信息

HiPrep is a trademark of Cytiva
HisPrep is a trademark of Cytiva
Sepharose is a trademark of Cytiva

象形图

Environment
GHS09

警示用语：

Warning

危险声明

H226

储存分类代码

3 - Flammable liquids

法规信息

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商品

Purification or Removal of Proteins and Peptides

This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from Cytiva.

Performing a Purification and On-column Refolding of an Insoluble Histidine-tagged Protein

This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap™ FF columns and ÄKTAprime from Cytiva.

Converting from Flow Velocity to Volumetric Flow Rates

This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.

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实验方案

Ni Sepharose 6 Fast Flow for Protein Purification

Ni Sepharose 6 Fast Flow purifies histidine-tagged proteins efficiently, offering high cross-linked agarose beads with Ni2+ ions.

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

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