推荐产品
物料
polycarbonate housing
polyethersulfone membrane
无菌性
non-sterile
特点
wetted part: no
包装
pkg of 25 ea
制造商/商品名称
Cytiva 28932293
管尺寸
15 mL
工作体积
6 mL
孔径
3 kDa MWCO
一般描述
Vivaspin ultrafiltration spin columns are designed for fast, non-denaturing concentration and desalting of proteins and other biological samples. Membrane spin columns such as Vivaspin combine the principle of size exclusion with centrifugation to achieve rapid concentration and high recovery of target biomolecules in a single tube. An upper compartment containing sample is separated from the lower compartment by a semipermeable membrane with an appropriate MWCO value chosen to exclude molecules of interest. Centrifugation is applied to force solvent through the membrane, leaving a more concentrated sample in the upper chamber. The Vivaspin column design permits protocols for one-step biomolecule concentration, rapid sample recovery (reverse spin protocol), desalting, and buffer exchange.
特点和优势
- Ease-of-use: One-step sample concentration in a single tube, for minimal sample handling and reduced sample loss, as well as pH compatibility over a broad range – pH 1 to 9.
- Sample protection: Patented Vivaspin dead-stop technology ensures samples cannot be concentrated to dryness and enables direct concentrate recovery.
- Ease-of-use: One-step sample concentration in a single tube, for minimal sample handling and reduced sample loss.
- High flow rates: And minimal membrane blockage through the vertical polyethersulfone (PES) membrane design.
- Minimal protein adsorption: Thanks to the Vivaspin™ polycarbonate tube design.
- Wide selection: Vivaspin sample concentrators are suitable for sample volumes ranging from 100 μL to 20 mL, with a range of molecular weight cut-off (MWCO) values from Mr 3000 to 100 000.
储存及稳定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()
储存分类代码
13 - Non Combustible Solids
法规信息
新产品
商品
This page describes troubleshooting strategies in purification of GST-tagged proteins using Cytiva products.
实验方案
Rules and good practice in sample preparation for Western blot sample preparation from cell culture and tissue samples.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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