选择尺寸
关于此项目
material
polypropylene and polyethylene resin, resin (Sephadex G-25)
sterility
non-sterile
feature
wetted part: no
packaging
pkg of 50 ea (columns)
manufacturer/tradename
Cytiva 28-9180-08
storage condition
dry media
bed size
13 mm × 26 mm
bed volume
3.5 mL
column I.D.
13 mm
working volume
0.5—1 mL
matrix
cross-linked dextran
particle size
>50 μm (dry), 85-260 μm
pore size
5000 Da MWCO
cleaning
2-13
working range
2-13
suitability
suitable for bioprocess medium
General description
PD MidiTrap G-25 is designed for convenient desalting and buffer exchange of biological samples and volumes up to 1.0 mL can be handled. Two alternative application protocols are available, gravity or centrifugation. By using the gravity protocol, a simple cleanup of the sample is done with one or several columns in parallel without any need for a purification system. With the centrifugation protocol, the samples are run in parallel in a standard centrifuge with minimal dilution of the eluted sample. Four adapters are included in each product pack to enable easy centrifugation.
Application
Features and Benefits
- Reliable and reproducible cleanup of proteins and other large biomolecules (>5000 Mr).
- Range of applications such as desalting, buffer exchange, and removal of low-molecular weight compounds.
- High desalting capacity.
- Flexible protocols with ability to use gravity flow or centrifugation.
- Sample preparation in the range of 0.5 to 1.0 mL for gravity and 0.75 to 1.0 mL with centrifugation.
Preparation Note
signalword
Warning
hcodes
存储类别
12 - Non Combustible Liquids
法规信息
商品
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.
实验室规模的脱盐是一种成熟、简单、快速的方法,可以在将样品转移至所需缓冲液的同时快速去除低分子量污染物。
实验方案
Rules and good practice in sample preparation for Western blot sample preparation from cell culture and tissue samples.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持