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安全信息

GE28-9031-32

Protein A High Performance Spintrap™

Cytiva 28-9031-32, pack of 16 columns

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UNSPSC代码:
41106500
NACRES:
NA.25

ligand

native protein A

保质期

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

特点

wetted part: no

包装

pack of 16 columns

制造商/商品名称

Cytiva 28-9031-32

储存条件

(20% Ethanol)

基质

highly cross-linked 6% agarose

平均零件尺寸

34 μm

cleaning

2-10(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)

working range

3-9

容量

>1 mg binding capacity(human IgG well)

储存温度

2-8°C

相关类别

一般描述

protein A HP SpinTrap™ are designed for the enrichment of proteins of interest, immunoprecipitation and the purification of monoclonal and polyclonal antibodies from cell lysates and biological fluids. Contain protein A Sepharose™ HP.

应用

Protein A Sepharose™ HP has high affinity for the Fc region of IgG from a variety of species. The two options in the Protein enrichment protocol allow the attached antigen (Protein of interest) to be eluted separately (cross-link protocol) or together with the antibody (classic protocol). There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.

There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.

特点和优势

  • For simple, small-scale antibody purification or enrichment of specific Proteins in prepacked, single-use spin columns that are ready to use.
  • Classic and cross-link protocols provide flexibility.
  • Elution conditions formatted for both electrophoresis and LC-MS analysis workflows.
  • Fast binding kinetics and high capacity provide high yield.
  • Prepacked with Protein A Sepharose™ High Performance for coupling of antibodies of IgG subclasses.
  • Easy scale-up with HiTrap® Protein A HP prepacked columns.

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
SpinTrap is a trademark of Cytiva

象形图

Flame
GHS02

警示用语：

Warning

危险声明

H226

法规信息

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商品

Purification Using Protein A-based Chromatography Media

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

Immunoprecipitation Techniques

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

实验方案

Performing a Purification of IgG Antibodies with Protein A HP SpinTrap™ columns

This page shows how to separate IgG antibodies by affinity chromatography using Protein A HP SpinTrap columns from Cytiva.

Performing a Purification of IgG Antibodies with Protein G HP SpinTrap™/Ab SpinTrap™

This page shows how to separate IgG antibodies by affinity chromatography using Protein G HP SpinTrap™/Ab SpinTrap™ from Cytiva.

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