Performing a Purification of IgG Antibodies with Protein A HP SpinTrap™ columns
Protein A HP SpinTrap™ (Figure 1) is a single-use spin column for protein enrichment of target antigens from antibody-antigen complexes and antibody purification from unclarified serum and cell culture supernatants. The columns are designed for small-scale purification of multiple samples in parallel and are suitable for use in antibody screening experiments. Protein A HP SpinTrap™ contains Protein A Sepharose® High Performance, which has high protein binding capacity, and is compatible with all buffers commonly used in antibody purification.
The 16 columns supplied in each package can be used with a standard microcentrifuge. Each package of Protein A HP SpinTrap™ contains alternative protocols for protein enrichment of target antibody-antigen complexes by immunoprecipitation and for antibody purification.
The same procedure as described for Protein G Sepharose® HP can be used for screening and purification of antibodies.
Figure 1.Protein A HP SpinTrap™ columns are used for protein enrichment of target proteins using antibodies bound to the protein A ligand or for small-scale purification of antibodies.
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