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安全信息

GE17-5442-02

Ficoll®-Paque Premium

Cytiva 17-5442-02, pack of 6 × 100 mL

别名:

Ficoll density gradient medium, cell culture media

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About This Item

UNSPSC代码:
12352201
NACRES:
NA.25

形式

liquid

包装

pack of 6 × 100 mL

制造商/商品名称

Cytiva 17-5442-02

颜色

Colorless to slightly yellow

pH值范围

5.5-7.5

相关类别

一般描述

Ficoll-Paque PREMIUM products are a range of sterile, ready-to-use density gradient media for the preparation of mononuclear cells.

Ficoll-Paque PREMIUM products are based on Ficoll-Paque PLUS (see Ficoll-Paque PLUS) which has been used for more than 30 yr as a sterile density medium for the isolation of high yields of mononuclear cells from bone marrow, peripheral blood, and cord blood. All Ficoll-Paque PREMIUM products differ from Ficoll-Paque PLUS in that they are manufactured under a Quality Management System certified to ISO 13485:2003.

应用

Optimized for the isolation of mononuclear cells from human peripheral blood, bone marrow, and umbilical cord blood.

Can be used for isolating a broad range of human mononuclear cells including those of a higher density and for separating blood cells from mice or rats.

Can be used when isolating lower-density human mononuclear cells (e.g., mesenchymal stromal cells or monocytes).

特点和优势

  • Sterile, ready to use reagents used to prepare mononuclear cell preparations from peripheral blood, bone marrow, and umbilical cord blood by density gradient centrifugation.
  • Manufactured under a Quality Management System certified to ISO 13485:2003.
  • Low levels of endotoxin (< 0.12 EU/mL) secured and tested.
  • Cytotoxicity grade 0.
  • A Regulatory Support File (RSF) is available for Ficoll-Paque PREMIUM products.

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

Ficoll is a registered trademark of Cytiva

法规信息

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Xin Ni et al.
PloS one, 12(9), e0184773-e0184773 (2017-09-15)
Inflammation has been shown to play an important role in the mechanisms involved in the pathogenesis of hypertension. Connexins (Cxs)-based gap junction channels (GJCs) or hemichannels (HCs) are involved in the maintenance of homeostasis in the immune system. However, the
Avanish K Varshney et al.
PloS one, 13(1), e0190537-e0190537 (2018-01-25)
Staphylococcus aureus can cause devastating and life-threatening infections. With the increase in multidrug resistant strains, novel therapies are needed. Limited success with active and passive immunization strategies have been attributed to S. aureus immune evasion. Here, we report on a

商品

Removal of Percoll after Centrifugation

This page shows methods to remove Percoll from a sample after centrifugation.

Properties of Mononuclear Cells Isolated by the Ficoll-Paque Method

The effects of the mononuclear cell separation method described by Bøyum procedure are noted below, since research situations may arise in which they are significant.

How to Fractionate and Analyze Gradients of Percoll

Density Marker Beads facilitate Percoll gradient monitoring in biological separations.

Applications of Percoll in Other Cell Types

The table was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll in various cell types.

实验方案

推荐单核细胞标准分离方法

以下操作步骤已接受Ficoll-Paque PLUS评估，推荐用于分离正常血样以尽可能提高可重现性。

Recommended Standard Method for Isolating Mononuclear Cells

The following procedure has been evaluated with Ficoll-Paque PLUS and is recommended for separation of normal blood samples for maximum reproducibility.

How to Make and Use Percoll Gradients

To prepare a Percoll gradient, the osmolality of Percoll must be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.

如何制作和使用Percoll梯度

为了配置Percoll梯度液，必须先用生理盐水或细胞培养基将Percoll调至生理性渗透压。

相关内容

Applications of Percoll In Blood Cells

Data was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll for a particular cell or tissue type.

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