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ligand
quaternary amine
描述
Ion Exchanger Type (value)
包装
pack of 300 mL
制造商/商品名称
Cytiva 17-5072-01
基质
6% cross-linked agarose with dextran surface extenders
粒径
45-165 μm
平均直径
90 μm
cleaning in place
2-14
工作范围
2-12
适用性
suitable for bioprocess medium
一般描述
Q Sepharose™ XL is based on a highly cross-linked, bead formed 6% agarose matrix similar to the well established Q Sepharose™ Fast Flow media. Dextran chains are covalently coupled to the agarose matrix and modified with quaternary ammonium (Q) strong anion exchange groups through chemically stable ether bonds. The stable agarose matrix and long, flexible chains of dextran with bound charged groups work together to increase loading capacity whilst allowing high flow rates.
With its high loading capacities and excellent physical and chemical stability, Q Sepharose™ XL is suitable for the capture stage of a downstream process, which is characterised by large loads of crude samples.
As member of the BioProcess media range, Q Sepharose™ XL meets industrial demands with security of supply and comprehensive technical and regulatory support.
特点和优势
- High loading capacities give more productive capture from clarified feedstocks.
- Strong anion exchanger designed for capture steps
- High chemical stability for effective CIP/sanitization
- The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool.
储存及稳定性
分析说明
法律信息
警示用语:
Warning
危险声明
储存分类代码
3 - Flammable liquids
法规信息
商品
This page shows Hydrophobic Interaction Chromatography (HIC) in a purification strategy.
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
实验方案
This page covers how and when to use Sepharose XL media for purification of proteins.
This page discusses how to do a purification with SOURCE™ media from Cytiva.
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
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