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安全信息

GE17-0727-20

SOURCE™ 15RPC

Cytiva 17-0727-20, pack of 10 mL

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UNSPSC代码:
13111000
NACRES:
NA.56

ligand

matrix surface

保质期

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

特点

autoclavable 20 min at 121°C

包装

pack of 10 mL

制造商/商品名称

Cytiva 17-0727-20

基质

polystyrene/divinylbenzene

平均零件尺寸

15 μm (monosized)

cleaning in place

1-14

working range

1-12

容量

~10 mg binding capacity(~10 mg BSA /ml medium at 300 cm/h, ~30 mg bacitracin/ml medium at 300 cm/h, ~50 mg insulin/ml medium at 300 cm/h)

适用性

suitable for bioprocess medium

相关类别

一般描述

SOURCE™ 15RPC is designed for reversed phase chromatography (RPC) with full pH range stability and suitable for polishing steps in industrial processes requiring high f low velocities and low back-pressure.

应用

SOURCE™ 15RPC is based on a 15 μm, monosized, rigid polystyrene/divinyl benzene matrix. This gives Low back pressures and high flow rates during operation allowing for high productivity. The 15 μm bead size allows for very high resolution chromatography steps.

High quality assurance standards in combination with the patented production method, particle structure is consistent both bead-to-bead and batch-to-batch.

A controlled pore size distribution and large specific surface area offer excellent capacity for a wide range of molecules, from small peptides and oligonucleotides up to large Proteins. With full pH range stability, SOURCE™ 15RPC is a strong alternative to silica-based RPC matrices.

SOURCE™ 15RPC is available in a range of different bulk pack sizes for easy scale-up and process development. As member of the BioProcess media range, SOURCE™ 15RPC meets industrial demands with security of supply and comprehensive technical and regulatory support.

特点和优势

  • Mono-sized 15 μm rigid beads gives Low back pressures and high flow rates allowing for high productivity.
  • Designed for high resolution reversed phase chromatography of Proteins, peptides and oligonucleotides.
  • High chemical stability allowing for wide range of working conditions with good resistance to cleaning conditions at high pH
  • BioProcess medium supported for industrial applications.

储存及稳定性

Store at 4 to 30 °C (20% Ethanol or 70% Acetonitrile)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

SOURCE is a trademark of Cytiva

象形图

Flame
GHS02

警示用语：

Warning

危险声明

H226

法规信息

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商品

Troubleshooting Reversed Phase Chromatography

“Ghosting” in chromatograms due to poor eluent quality; trace organic impurities concentrate, causing unknown peaks.

RPC Purification Options and Scale up with SOURCE™ Media

The different stages of working with SOURCE™ (Cytiva) for high-resolution separation are discussed, from purification to polishing and scaling up

Column Packing and Preparation for Hydrophobic Interaction and Reversed Phased Chromatography

This page discusses column packing and preparation techniques for reverse phase chromatography.

HIC as a Capture Step

This page shows Hydrophobic Interaction Chromatography (HIC) in a purification strategy.

实验方案

Reverse Phased Chromatography (RPC) in Practice

In this section the practical aspect of Reverse Phased Chromatography ( RPC) is discussed including media and column selection and eluent selection and preparation.

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